2nd Department of Medicine, Klinikum rechts der Isar, Technical University of Munich, Ismaningerstr. 22, 81675 Munich, Germany.
J Biophotonics. 2011 Jan;4(1-2):108-13. doi: 10.1002/jbio.201000028. Epub 2010 Jun 8.
Probe-based confocal laser scanning endomicroscopy (pCLE) enables in-vivo histopathology during ongoing endoscopy. The most frequently used fluorophore is fluorescein sodium administered intravenously. Despite the increased use of pCLE, there are hardly any data on the ideal fluorescein concentration. Therefore, rectal mucosa of pigs was examined after injection (i.v.) of fluorescein as a single bolus (0.1 ml/kg body weight) in different concentrations (0.5%, 1%, 2%, 5%, 10%). Video sequences were recorded after 1, 5 and 60 min. For objective evaluation signal-to-noise ratio (SNR) was computed. For subjective evaluation, video sequences were randomized and blindly evaluated by experienced endomicroscopists. In total, 19037 images were analyzed. The mean SNR increased from the lowest (0.5%; SNR 6.75, range 3.55) to the highest concentration (10%; SNR 9.11, range 3.18). Subjective evaluation demonstrated best image quality with fluorescein concentration of 5%. In conclusion, pCLE shows best results using single injection of IV fluorescein 5%.
基于探针的共聚焦激光扫描内镜检查（pCLE）可在内窥镜检查过程中实时进行体内组织病理学检查。最常使用的荧光团是静脉内注射的荧光素钠。尽管 pCLE 的使用越来越多，但关于理想的荧光素浓度几乎没有任何数据。因此，在不同浓度（0.5％，1％，2％，5％，10％）下，对猪的直肠粘膜进行了注射（静脉内）单剂量（0.1ml / kg 体重）后检查荧光素。在 1、5 和 60 分钟后记录视频序列。为了进行客观评估，计算了信噪比（SNR）。为了进行主观评估，将视频序列随机化并由经验丰富的内镜医生进行盲法评估。总共分析了 19037 张图像。平均 SNR 从最低浓度（0.5％；SNR 6.75，范围 3.55）增加到最高浓度（10％；SNR 9.11，范围 3.18）。主观评估表明，荧光素浓度为 5％时图像质量最佳。总之，pCLE 使用单次静脉内注射 5％的荧光素可获得最佳结果。
