结直肠癌中与肿瘤分期和分级相关的疏水性蛋白。
Hydrophobic protein in colorectal cancer in relation to tumor stages and grades.
机构信息
School of Pharmaceutical Sciences, Universiti Sains Malaysia, USM 11800, Penang, Malaysia.
出版信息
World J Gastroenterol. 2010 Jun 14;16(22):2754-63. doi: 10.3748/wjg.v16.i22.2754.
AIM
To identify differentially expressed hydrophobic proteins in colorectal cancer.
METHODS
Eighteen pairs of colorectal cancerous tissues in addition to tissues from normal mucosa were analysed. Hydrophobic proteins were extracted from the tissues, separated using 2-D gel electrophoresis and analysed using Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS). Statistical analysis of the proteins was carried out in order to determine the significance of each protein to colorectal cancer (CRC) and also their relation to CRC stages, grades and patients' gender.
RESULTS
Thirteen differentially expressed proteins which were expressed abundantly in either cancerous or normal tissues were identified. A number of these proteins were found to relate strongly with a particular stage or grade of CRC. In addition, the association of these proteins with patient gender also appeared to be significant.
CONCLUSION
Stomatin-like protein 2 was found to be a promising biomarker for CRC, especially in female patients. The differentially expressed proteins identified were associated with CRC and may act as drug target candidates.
目的
鉴定结直肠癌中差异表达的疏水性蛋白。
方法
分析了 18 对结直肠癌组织以及正常黏膜组织。从组织中提取疏水性蛋白，使用二维凝胶电泳分离，并使用液相色谱串联质谱（LC/MS/MS）进行分析。对蛋白质进行统计学分析，以确定每种蛋白质对结直肠癌（CRC）的显著性，并确定它们与 CRC 分期、分级和患者性别之间的关系。
结果
鉴定出 13 种在癌组织或正常组织中表达丰富的差异表达蛋白。其中一些蛋白与 CRC 的特定分期或分级密切相关。此外，这些蛋白与患者性别之间的关联似乎也具有显著性。
结论
发现 STOM 样蛋白 2 是 CRC 有前途的生物标志物，特别是在女性患者中。鉴定出的差异表达蛋白与 CRC 相关，可能作为药物靶标候选物。
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