Bone Regeneration Laboratory, Department of Muskulo-Skeletal Oncology, Research Institute Codivilla-Putti, Rizzoli Orthopaedic Institute, Bologna, Italy.
Tissue Eng Part A. 2010 Sep;16(9):2967-76. doi: 10.1089/ten.tea.2009.0637.
Limited incorporation and modest bone remodeling can cause allograft failure. We investigated whether mesenchymal stem cells (MSCs) and osteogenic protein-1 (OP-1) can improve allograft integration. A 3-cm full-size intercalary bone defect was created in the mid-diaphysis of the metatarsal bone of the sheep and it was replaced with an allograft alone (control group), or with MSCs (MSC group), OP-1 (OP-1 group), or MSCs and OP-1 (MSC + OP-1 group). Radiographic results showed a faster and complete integration of the allograft in the MSC + OP-1 group. Histology demonstrated that the amount of new bone was significantly greater inside the graft and a longer vessel penetration in the MSC + OP-1 group than in others. Mechanical strength of the allograft was not compromised by the high rate of bone remodeling. These results demonstrated that the association of MSCs and OP-1 improve bone allograft integration promoting an almost complete bone restoring.
有限的整合和适度的骨重塑可能导致移植物失败。我们研究了间充质干细胞（MSCs）和骨形成蛋白-1（OP-1）是否可以改善移植物的整合。在羊的跖骨骨干中段创建了一个 3 厘米的全尺寸节段性骨缺损，并用单独的移植物（对照组）、MSCs（MSC 组）、OP-1（OP-1 组）或 MSCs 和 OP-1（MSC+OP-1 组）进行替代。放射学结果显示 MSC+OP-1 组的移植物更快、更完全的整合。组织学显示，MSC+OP-1 组移植物内的新骨量明显增加，血管穿透更长。高骨重塑率并没有影响移植物的机械强度。这些结果表明，MSC 和 OP-1 的联合使用可以改善骨移植物的整合，促进几乎完全的骨修复。
