Area de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, E-24071 León, Spain.
Mol Plant. 2010 Sep;3(5):842-53. doi: 10.1093/mp/ssq027. Epub 2010 Jun 9.
The biochemical and molecular processes involved in the habituation of maize cells to growth in the presence of the cellulose biosynthesis inhibitor dichlobenil (DCB) were investigated. DCB affects the synthesis of cellulose both in active and stationary growth phases and alters the expression of several CesA genes. Of these, ZmCesA5 and ZmCesA7 seem to play a major role in habituating cells to growth in the presence of DCB. As a consequence of the reduction in cellulose, the expression of several genes involved in the synthesis of hydroxycinnamates is increased, resulting in cell walls with higher levels of ferulic and p-coumaric acids. A proteomic analysis revealed that habituation to DCB is linked to modifications in several metabolic pathways. Finally, habituated cells present a reduction in glutathione S-transferase detoxifying activity and antioxidant activities. Plant cell adaptation to the disturbance of such a crucial process as cellulose biosynthesis requires changes in several metabolic networks, in order to modify cell wall architecture and metabolism, and survive in the presence of the inhibitor. Some of these modifications are described in this paper.
本研究调查了玉米细胞在纤维素生物合成抑制剂二氯苯醚（DCB）存在的情况下适应生长的生化和分子过程。DCB 影响纤维素的合成，无论是在活跃生长阶段还是静止生长阶段，并改变了几个 CesA 基因的表达。其中，ZmCesA5 和 ZmCesA7 似乎在使细胞适应 DCB 存在下的生长中起主要作用。由于纤维素的减少，参与羟基肉桂酸合成的几个基因的表达增加，导致细胞壁中含有更高水平的阿魏酸和对香豆酸。蛋白质组学分析表明，对 DCB 的适应与几个代谢途径的变化有关。最后，适应 DCB 的细胞表现出谷胱甘肽 S-转移酶解毒活性和抗氧化活性的降低。植物细胞适应纤维素生物合成这一关键过程的干扰需要改变几个代谢网络，以改变细胞壁结构和代谢，并在抑制剂存在的情况下存活。本文描述了其中的一些变化。
