Área de Fisiología Vegetal, Facultad de CC. Biológicas y Ambientales, Universidad de León, León, Spain.
Plant Signal Behav. 2011 Jan;6(1):143-6. doi: 10.4161/psb.6.1.14304. Epub 2011 Jan 1.
Cellulose biosynthesis inhibitors, such as dichlobenil (DCB), have become a valuable tool for the analysis of structural and compositional plasticity of plant cell walls. By stepwise increasing the concentration of DCB in the culture medium, we obtained maize cells able to cope with DCB through the acquisition of a modified cell wall in which cellulose was partially replaced by a more extensive network of feruloylated arabinoxylans. Recently we demonstrated that the expression of several Cellulose Synthase and phenylpropanoid-related genes is altered in DCB-habituated cells. In addition, by using a proteomic approach we identified several proteins induced or repressed in DCB-habituated cells. After a more in-depth analysis, some new proteins induced (two inhibitors TAXI-IV, an α-1,4-glucan-protein synthase, and a pectinesterase inhibitor) or repressed (a chaperonin 60, a fructokinase-1 and a spermidine synthase 1) were identified, and their possible role in the context of DCB-habituation is discussed.
纤维素生物合成抑制剂，如二氯苯醚（DCB），已成为分析植物细胞壁结构和组成可塑性的有用工具。通过逐步增加培养基中 DCB 的浓度，我们获得了能够通过获得经过修饰的细胞壁来应对 DCB 的玉米细胞，其中纤维素部分被更广泛的阿魏酰阿拉伯木聚糖网络取代。最近我们证明，在适应 DCB 的细胞中，几个纤维素合酶和苯丙烷相关基因的表达发生了改变。此外，通过使用蛋白质组学方法，我们鉴定了在适应 DCB 的细胞中诱导或抑制的几种蛋白质。经过更深入的分析，鉴定出一些新诱导的蛋白质（两种抑制剂 TAXI-IV、一种α-1,4-葡聚糖蛋白合酶和一种果胶甲酯酶抑制剂）或抑制的蛋白质（一种热休克蛋白 60、一种果糖激酶-1 和一种亚精胺合酶 1），并讨论了它们在适应 DCB 背景下的可能作用。
