组胺诱导的HeLa细胞中肌醇磷脂水解的特征
Characteristics of histamine-induced inositol phospholipid hydrolysis in HeLa cells.
作者信息
Bristow D R, Young J M
机构信息
Department of Pharmacology, University of Cambridge, United Kingdom.
出版信息
Agents Actions Suppl. 1991;33:387-92. doi: 10.1007/978-3-0348-7309-3_28.
PMID:2053516
Abstract
HeLa cells, a human epithelial carcinoma, contain Histamine H1-receptors, as defined by [3H] mepyramine binding studies. Histamine, via H1-receptors, stimulates the production of inositol phosphates (IP) with an EC50 of 1.2 microM. Preincubation of HeLa cells with histamine, followed by a further histamine challenge results in a slower time course of [3H] IP production, suggesting the occurrence of H1-receptor desensitisation.
摘要
人上皮癌细胞系HeLa细胞含有组胺H1受体，这是通过[3H]美吡拉敏结合研究确定的。组胺通过H1受体刺激肌醇磷酸（IP）的产生，其半数有效浓度（EC50）为1.2微摩尔。用组胺对HeLa细胞进行预孵育，随后再次用组胺刺激，会导致[3H] IP产生的时间进程变慢，这表明发生了H1受体脱敏。
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