Departamento de Neurofisiologia, INR, SSA, Deleg. Tlalpan, Mexico City, Mexico.
Neurochem Res. 2010 Oct;35(10):1538-45. doi: 10.1007/s11064-010-0213-4. Epub 2010 Jun 10.
The sensorimotor cortex and the cerebellum are interconnected by the corticopontocerebellar (CPC) pathway and by neuronal groups such as the serotonergic system. Our aims were to determine the levels of cerebellar serotonin (5-HT) and lipid peroxidation (LP) after cortical iron injection and to analyze the motor function produced by the injury. Rats were divided into the following three groups: control, injured and recovering. Motor function was evaluated using the beam-walking test as an assessment of overall locomotor function and the footprint test as an assessment of gait. We also determined the levels of 5-HT and LP two and twenty days post-lesion. We found an increase in cerebellar 5-HT and a concomitant increase in LP in the pons and cerebellum of injured rats, which correlated with their motor deficits. Recovering rats showed normal 5-HT and LP levels. The increase of 5-HT in injured rats could be a result of serotonergic axonal injury after cortical iron injection. The LP and motor deficits could be due to impairments in neuronal connectivity affecting the corticospinal and CPC tracts and dysmetric stride could be indicative of an ataxic gait that involves the cerebellum.
感觉运动皮层和小脑通过皮质脑桥小脑（CPC）通路以及 5-羟色胺能系统等神经元群相互连接。我们的目的是确定皮质铁注射后小脑 5-羟色胺（5-HT）和脂质过氧化（LP）的水平，并分析损伤引起的运动功能。大鼠分为以下三组：对照组、损伤组和恢复组。通过走棒试验评估整体运动功能，足迹试验评估步态，评估运动功能。我们还在损伤后 2 天和 20 天测定 5-HT 和 LP 的水平。我们发现损伤大鼠的小脑 5-HT 增加，桥脑和小脑的 LP 也随之增加，这与它们的运动缺陷相关。恢复组大鼠的 5-HT 和 LP 水平正常。损伤大鼠 5-HT 的增加可能是皮质铁注射后 5-羟色胺能轴突损伤的结果。LP 和运动缺陷可能是由于神经元连接受损，影响皮质脊髓和 CPC 束，步幅不均可能表明涉及小脑的共济失调步态。
