Laboratory of Functional and Metabolic Imaging, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Magn Reson Med. 2010 Aug;64(2):334-40. doi: 10.1002/mrm.22359.
Full signal intensity (1)H-[(13)C] NMR spectroscopy, combining a preceding (13)C-editing block based on an inversion BISEP (B(1)-insensitive spectral editing pulse) with a spin-echo coherence-based localization, was developed and implemented at 14.1 T. (13)C editing of the proposed scheme was achieved by turning on and off the (13)C adiabatic full passage in the (13)C-editing block to prepare inverted and noninverted (13)C-coupled (1)H coherences along the longitudinal axis prior to localization. The novel (1)H-[(13)C] NMR approach was applied in vivo under infusion of the glia-specific substrate [2-(13)C] acetate. Besides a approximately 50% improvement in sensitivity, spectral dispersion was enhanced at 14.1 T, especially for J-coupled metabolites such as glutamate and glutamine. A more distinct spectral structure at 1.9-2.2 ppm(parts per million) was observed, e.g., glutamate C3 showed a doublet pattern in both simulated (1)H spectrum and in vivo (13)C-edited (1)H NMR spectra. Besides (13)C time courses of glutamate C4 and glutamine C4, the time courses of glutamate C3 and glutamine C3 obtained by (1)H-[(13)C] NMR spectroscopy were reported for the first time. Such capability should greatly improve the ability to study neuron-glial metabolism using (1)H-observed (13)C-edited NMR spectroscopy.
全信号强度 (1)H-[(13)C]NMR 波谱学，结合了基于反转 BISEP（B(1)- 不敏感谱编辑脉冲）的 (13)C-编辑模块和基于自旋回波相干的定位，在 14.1T 下进行了开发和实施。所提出方案的 (13)C 编辑是通过在 (13)C-编辑模块中打开和关闭 (13)C 绝热全通过来实现的，以便在定位之前沿纵轴制备反转和未反转 (13)C 耦合 (1)H 相干。该新型 (1)H-[(13)C]NMR 方法在神经胶质特异性底物 [2-(13)C]乙酸盐输注下进行了体内应用。除了灵敏度提高约 50%之外，在 14.1T 下还增强了光谱色散，特别是对于谷氨酸和谷氨酰胺等 J 耦合代谢物。在 1.9-2.2ppm（百万分之一）处观察到更明显的光谱结构，例如，谷氨酸 C3 在模拟 (1)H 光谱和体内 (13)C 编辑 (1)H NMR 光谱中均显示出双峰模式。除了谷氨酸 C4 和谷氨酰胺 C4 的 (13)C 时间历程外，首次报道了通过 (1)H-[(13)C]NMR 光谱获得的谷氨酸 C3 和谷氨酰胺 C3 的时间历程。这种能力应该大大提高使用 (1)H 观察的 (13)C 编辑 NMR 光谱研究神经元-神经胶质代谢的能力。
