Division of Laboratory for Probiotic Research, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.
Microbiol Immunol. 2010 May;54(5):265-75. doi: 10.1111/j.1348-0421.2010.00224.x.
The anti-infectious activity of synbiotics against methicillin-resistant Staphylococcus aureus (MRSA) infection was evaluated using a novel lethal mouse model. Groups of 12 mice treated with multiple antibiotics were infected orally with a clinical isolate of MRSA at an inoculum of 10(8) CFU on day 7 after starting the antibiotics. A dose of 400 mg/kg 5-fluorouracil (5-FU) was injected intraperitoneally on day 7 after the infection. A dose of 10(8) CFU Bifidobacterium breve strain Yakult and 10 mg of galactooligosaccharides (GOS) were given orally to mice daily with the antibiotic treatment until day 28. The intestinal population levels of MRSA in the mice on multiple antibiotics were maintained stably at 10(8) CFU/g of intestinal contents after oral MRSA infection and the subsequent 5-FU treatment killed all the mice in the group within 14 days. B. breve administration saved most of the mice, but the synbiotic treatment saved all of the mice from lethal MRSA infection. The synbiotic treatment was effective for the treatment of intestinal infection caused by four MRSA strains with different toxin productions. There was a large difference among the six Bifidobacteria strains that were naturally resistant to the antibacterial drugs used. B. breve in combination with GOS is demonstrated to have valuable preventive and curative effects against even fatal MRSA infections.
采用新型致死性小鼠模型评价了合生剂对耐甲氧西林金黄色葡萄球菌（MRSA）感染的抗感染活性。在开始使用抗生素 7 天后，用 10(8) CFU 的临床分离 MRSA 对接受多种抗生素治疗的 12 只小鼠进行经口感染。在感染后 7 天，用 400 mg/kg 的 5-氟尿嘧啶（5-FU）经腹腔注射。在抗生素治疗期间，每天给小鼠口服 10(8) CFU 的短双歧杆菌 Yakult 菌株和 10 mg 的半乳糖寡糖（GOS）。经口 MRSA 感染和随后的 5-FU 治疗后，接受多种抗生素治疗的小鼠肠道内的 MRSA 种群水平稳定维持在 10(8) CFU/g 肠道内容物，在 14 天内杀死了该组中的所有小鼠。B. breve 的给药挽救了大多数小鼠，但合生剂治疗使所有小鼠免受致命性 MRSA 感染。合生剂治疗对由四种具有不同毒素产生的 MRSA 菌株引起的肠道感染有效。在对所用抗菌药物天然耐药的六种双歧杆菌菌株之间存在很大差异。证明短双歧杆菌与 GOS 联合具有有价值的预防和治疗作用，甚至可对抗致命性的 MRSA 感染。
