Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Square 2, 16206 Prague 6, Czech Republic.
J Biomater Sci Polym Ed. 2010;21(12):1669-85. doi: 10.1163/092050609X12547435571131. Epub 2010 Jun 9.
N-(2-Hydroxypropyl)methacrylamide (HPMA) co-polymers containing disulfide and carbonyl thiazolidine-2-thione (TT) reactive groups in their side-chains (pHPMA-TT) were used as reductively removable chemical modification of the surface of cowpea mosaic viruses (CPMV). CPMV was used in this study as a model particle for viral gene delivery. The co-polymer reaction with CPMV surfaces carried out in aqueous solution was evaluated by monitoring the changes in the weight-average molecular weight and hydrodynamic size of the viruses using light scattering methods. The reaction conditions were optimized. The surface modification of CPMV with pHPMA-TT under selected conditions (concentrations of a coating polymer (c(p)) and NaCl) has not influenced the size distribution of the viral particles. The uncharged polymers bound to the viral surface via biodegradable S-S bonds can be fully removed by the exchange reaction with reductive dithiothreitol. To achieve optimal covering of viral surfaces, the positively charged reactive polymers (with or without biodegradable S-S bonds) should be applied at low concentrations (c(p)=0.1-0.5 mg/ml) and in presence of NaCl (0.15 M).
N-(2-羟丙基)甲基丙烯酰胺（HPMA）共聚物在其侧链中含有二硫键和羰基噻唑啉-2-硫酮（TT）反应基团（pHPMA-TT），被用作牛痘病毒（CPMV）表面的还原性可去除化学修饰。CPMV 在此研究中被用作病毒基因传递的模型颗粒。通过使用光散射方法监测病毒的重均分子量和流体力学尺寸的变化，评估了在水溶液中与 CPMV 表面的共聚物反应。优化了反应条件。在选定的条件（涂层聚合物浓度（c(p)）和 NaCl）下，用 pHPMA-TT 对 CPMV 进行表面修饰，不会影响病毒颗粒的尺寸分布。通过与还原性二硫苏糖醇的交换反应，可以完全去除与病毒表面结合的非带电聚合物的生物可降解 S-S 键。为了实现病毒表面的最佳覆盖，应在低浓度（c(p)=0.1-0.5mg/ml）和 NaCl（0.15M）存在下，应用带正电荷的反应性聚合物（具有或不具有生物可降解的 S-S 键）。
