Laboratorio de Neurofisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, Guadalajara, Jalisco, México.
J Food Prot. 2010 Jun;73(6):1121-3. doi: 10.4315/0362-028x-73.6.1121.
Toxoplasmosis is an infection caused by Toxoplasma gondii, an intracellular obligate parasite. Its transmission has usually been attributed to ingestion of undercooked or raw meat. The frequency of T. gondii in pork, the most common meat for human consumption in Jalisco, Mexico, is unknown; in Guadalajara city high prevalence of human toxoplasmosis has been documented. Forty-eight samples of pork meat from butcher shops in Ocotlán city were analyzed. Through bioassay, 50 g of tissue was homogenized in an acidic pepsin solution and inoculated subcutaneously to previously immunosuppressed mice. Blood samples from the mice tail vein were obtained before inoculation and 7, 14, 28, and 45 days postinoculation to analyze anti-Toxoplasma immunoglobulin (Ig) M and IgG antibody kinetics by indirect enzyme-linked immunosorbent assay. For histopathology, small fragments of the brain, lungs, heart, and skeletal muscle were extracted on day 45 and were stained with hematoxylin and eosin. Also, DNA was extracted from the pork meat for PCR amplification of the B1 gene. Even though all pork samples were negative by histopathology and PCR, IgG and IgM antibodies against T. gondii were detected in 1 of the 48 inoculated mice, reflecting a frequency of 2.1% positive pork meat, which is lower than expected but similar to that found in other regions.
弓形虫病是由刚地弓形虫引起的感染，刚地弓形虫是一种细胞内专性寄生虫。其传播通常归因于摄入未煮熟或生的肉类。墨西哥哈利斯科州最常见的人类食用肉类——猪肉中刚地弓形虫的频率尚不清楚；在瓜达拉哈拉市，已记录到人类弓形虫病的高患病率。分析了奥科特兰市肉店的 48 份猪肉样本。通过生物测定，将 50 克组织在酸性胃蛋白酶溶液中匀浆，并皮下接种给先前免疫抑制的小鼠。在接种前和接种后 7、14、28 和 45 天从小鼠尾静脉采集血样，通过间接酶联免疫吸附试验分析抗弓形虫免疫球蛋白（Ig）M 和 IgG 抗体动力学。对于组织病理学，在第 45 天从小鼠的脑、肺、心脏和骨骼肌中提取小块组织，并使用苏木精和伊红染色。此外，从猪肉中提取 DNA 进行 B1 基因的 PCR 扩增。尽管所有猪肉样本在组织病理学和 PCR 检查中均为阴性，但在 48 只接种的小鼠中有 1 只检测到针对弓形虫的 IgG 和 IgM 抗体，反映出阳性猪肉的频率为 2.1%，这低于预期，但与其他地区的发现相似。
