Iqbal Asma, Janecko Nicol, Pollari Frank, Dixon Brent
Bureau of Microbial Hazards, Food Directorate, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON K1A 0K9, Canada.
Centre for Food-borne, Environmental and Zoonotic Infectious Diseases, Public Health Agency of Canada, 255 Woodlawn Road West, Unit 120, Guelph, ON N1H 8J1, Canada.
Food Waterborne Parasitol. 2018 Nov 8;13:e00031. doi: 10.1016/j.fawpar.2018.e00031. eCollection 2018 Dec.
is a protozoan parasite which infects a wide variety of mammals and birds worldwide, including humans. Human toxoplasmosis is primarily transmitted through the ingestion of tissue cysts in raw or poorly cooked meat and organs of infected animals, or through the ingestion of oocysts in food, water or soil contaminated with cat faeces. There is a distinct paucity of information on the prevalence and molecular characteristics of in retail meats in Canada. This study reports the presence of DNA in 4.3% (12 of 281) of packages of fresh ground beef, chicken breasts and ground pork purchased at retail in three Canadian provinces. prevalence was very similar among all three meat types tested, and among the provinces sampled. Genotyping of positive samples by means of PCR-RFLP and DNA sequencing demonstrated the presence of both type II (66.7%) and type III (33.3%). These findings provide baseline data on the prevalence of DNA in fresh meats purchased at retail in Canada and will allow for more accurate and meaningful health risk assessments for the purposes of developing food safety guidelines and policies.
是一种原生动物寄生虫，在全球范围内感染包括人类在内的多种哺乳动物和鸟类。人类弓形虫病主要通过摄入受感染动物的生肉或未煮熟的肉及器官中的组织囊肿，或通过摄入被猫粪便污染的食物、水或土壤中的卵囊传播。关于加拿大零售肉类中弓形虫的流行情况和分子特征，明显缺乏相关信息。本研究报告称，在加拿大三个省份零售购买的281包新鲜绞碎牛肉、鸡胸肉和绞碎猪肉中，有4.3%（12包）检测出弓形虫DNA。在所有三种测试肉类类型以及抽样省份中，弓形虫的流行情况非常相似。通过PCR-RFLP和DNA测序对阳性样本进行基因分型，结果显示存在II型（66.7%）和III型（33.3%）弓形虫。这些发现提供了加拿大零售购买的新鲜肉类中弓形虫DNA流行情况的基线数据，将有助于为制定食品安全指南和政策进行更准确、更有意义的健康风险评估。
