肿瘤坏死因子-α -308 G/A多态性对突尼斯丙型肝炎病毒（HCV）感染患者肝纤维化严重程度无影响。
Lack of effect of tumor necrosis factor-alpha -308 G/A polymorphism on severity of liver fibrosis in Tunisian hepatitis C virus (HCV)-infected patients.
作者信息
Bouzgarrou N, Hassen E, Gabbouj S, Schvoerer E, Ben Mami N, Triki H, Chouchane L
机构信息
Molecular Immuno-Oncology Laboratory, Faculty of Medicine, 5019 Monastir, Tunisia.
出版信息
Gastroenterol Clin Biol. 2010 Apr-May;34(4-5):297-304. doi: 10.1016/j.gcb.2010.03.008. Epub 2010 May 27.
OBJECTIVES
Tumor necrosis factor alpha (TNF-alpha) plays a key role in the immune response. An elevated plasma level of TNF-alpha was repeatedly observed in patients with active liver injury or cirrhosis regardless of the aetiology. The G/A transition at position -308 in the promoter region have been shown to influence TNF-alpha expression. In this study, we aimed to evaluate the impact of TNF-alpha -308 G/A functional polymorphism on fibrosis severity in Tunisian Hepatitis C Virus (HCV)-infected patients.
METHODS
TNF-alpha -308 G/A polymorphism was evaluated by polymerase chain reaction (PCR) amplification followed by Restriction Fragment Length Polymorphism (RFLP) method in 53 chronic hepatitis C patients. Single-nucleotide polymorphism (SNP) frequencies were compared with regard to liver fibrosis severity as assessed by the METAVIR scoring system (F1-F2; n=22 versus F3-F4; n=31).
RESULTS
The genotype distribution of the TNF-alpha -308 G/A polymorphism among the HCV-infected patients was as follows : GG : 67.9%, GA : 32.1%, AA : 0%. With regard to fibrosis score, no significant differences in TNF-alpha genotype distribution were observed between F1-F2 and F3-F4 patients (p=0.15).
CONCLUSION
No significant association between TNF-alpha -308 polymorphism and and the severity of liver fibrosis was found in our Tunisian cohort.
目的
肿瘤坏死因子α（TNF-α）在免疫反应中起关键作用。无论病因如何，在活动性肝损伤或肝硬化患者中均反复观察到血浆TNF-α水平升高。已证明启动子区域-308位的G/A转换会影响TNF-α的表达。在本研究中，我们旨在评估TNF-α -308 G/A功能多态性对突尼斯丙型肝炎病毒（HCV）感染患者肝纤维化严重程度的影响。
方法
采用聚合酶链反应（PCR）扩增后限制性片段长度多态性（RFLP）方法，对53例慢性丙型肝炎患者的TNF-α -308 G/A多态性进行评估。根据METAVIR评分系统（F1-F2；n=22对F3-F4；n=31）评估肝纤维化严重程度，比较单核苷酸多态性（SNP）频率。
结果
HCV感染患者中TNF-α -308 G/A多态性的基因型分布如下：GG：67.9%，GA：32.1%，AA：0%。关于纤维化评分，F1-F2和F3-F4患者之间在TNF-α基因型分布上未观察到显著差异（p=0.15）。
结论
在我们的突尼斯队列中，未发现TNF-α -308多态性与肝纤维化严重程度之间存在显著关联。
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