Department of Anatomy and Brain Science, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan.
Biochem Biophys Res Commun. 2010 Jul 16;398(1):19-25. doi: 10.1016/j.bbrc.2010.05.162. Epub 2010 Jun 9.
Emerging evidence suggests that GPR155, an integral membrane protein related to G-protein coupled receptors, has specific roles in Huntington disease and autism spectrum disorders. This study reports the structural organization of mouse GPR155 gene and the generation of five variants (Variants 1-5) of GPR155 mRNA, including so far unknown four variants. Further, it presents the level of expression of GPR155 mRNA in different mouse tissues. The mRNAs for GPR155 are widely expressed in adult mouse tissues and during development. In situ hybridization was used to determine the distribution of GPR155 in mouse brain. The GPR155 mRNAs are widely distributed in forebrain regions and have more restricted distribution in the midbrain and hindbrain regions. The highest level of expression was in the lateral part of striatum and hippocampus. The expression pattern of GPR155 mRNAs in mouse striatum was very similar to that of cannabinoid receptor type 1. The predicted protein secondary structure indicated that GPR155 is a 17-TM protein, and Variant 1 and Variant 5 proteins have an intracellular, conserved DEP domain near the C-terminal.
新出现的证据表明，GPR155 是一种与 G 蛋白偶联受体相关的完整膜蛋白，它在亨廷顿病和自闭症谱系障碍中具有特定的作用。本研究报告了小鼠 GPR155 基因的结构组织和 GPR155 mRNA 的五个变体（变体 1-5）的产生，包括迄今为止未知的四个变体。此外，它还介绍了 GPR155 mRNA 在不同小鼠组织中的表达水平。GPR155 的 mRNAs 在成年小鼠组织和发育过程中广泛表达。原位杂交用于确定 GPR155 在小鼠大脑中的分布。GPR155 mRNAs 在大脑前区广泛分布，在中脑和后脑区分布更为局限。表达水平最高的是纹状体和海马体的外侧部分。GPR155 mRNAs 在小鼠纹状体中的表达模式与大麻素受体 1 非常相似。预测的蛋白质二级结构表明 GPR155 是一种 17-TM 蛋白，变体 1 和变体 5 蛋白在 C 端附近具有细胞内保守的 DEP 结构域。
