肥大细胞类胰蛋白酶在瘢痕中的表达
[Expression of mast cell tryptase in scar].
作者信息
Gao Feng, Zhao Yang, Feng Yong-qiang, Huo Ran, Xue Wen-jun, Wang Fa-gang, Lv Ren-rong, Xue Feng, Li Qiang, Zhang Jian
机构信息
Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2010 Mar;26(2):132-5.
OBJECTIVE
To investigate the expression and distribution of mast cell tryptase (MCT) in scar, and to discuss the different MCT gene expression in keloid, hypertrophic scar and normal skin.
METHODS
20 samples of keloid, 20 samples of hypertrophic scar and 20 samples of normal skin were collected. The distribution of MCT was investigated by immunofluorescence histochemistry, and the MCT mRNA expression was detected by Relative Quantification real-time fluorescent PCR.
RESULTS
MCT gene was mainly located in the collagen fiber bundles of the scar, especially in the superficial layer of scar. MCT mRNA expression was significantly higher in keloid than that in hypertrophic scar and normal skin (P < 0.01). Averagely, the MCT gene expression in keloid was 2.5 times and 5.4 times of that in hypertrophic scar and normal skin.
CONCLUSIONS
MCT gene may play a role in the pathogenesis of scar.
目的
研究肥大细胞类胰蛋白酶（MCT）在瘢痕中的表达及分布情况，并探讨瘢痕疙瘩、增生性瘢痕和正常皮肤中MCT基因表达的差异。
方法
收集20例瘢痕疙瘩标本、20例增生性瘢痕标本和20例正常皮肤标本。采用免疫荧光组织化学法研究MCT的分布，采用相对定量实时荧光PCR法检测MCT mRNA的表达。
结果
MCT基因主要位于瘢痕的胶原纤维束中，尤其是瘢痕的表层。瘢痕疙瘩中MCT mRNA的表达明显高于增生性瘢痕和正常皮肤（P < 0.01）。瘢痕疙瘩中MCT基因的表达平均是增生性瘢痕和正常皮肤的2.5倍和5.4倍。
结论
MCT基因可能在瘢痕的发病机制中起作用。
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