Wu Wen-yi, Zhu Shi-ze, Wu Rui-lan, Zou Cheng-hao, Wang Zhao-yang, Huang Zhong-xin
Departent of Plastic Surgery, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2005 Nov;21(6):448-51.
To study the expression of Skp2 gene (s-phase kinase associated protein 2) in the pathological scars and its relationship with p27kip1 level and to investigate its role and its probable mechanism in the pathogenesis of abnormal scars.
Immunohistochemical technique was performed to detect the expression and distribution of Skp2 and p27kip1 protein in hypertrophic scar (42 cases), keloid (18 cases), normal scar (40 cases) and normal skin (50 cases), statistics was used to analyze the data.
The positive rate of Skp2 and p27kip1 protein expression was not statistically different between the hypertrophic scar and keloid (P > 0.05), while they were all remarkably significant in comparison between normal scar and abnormal scar (P < 0.05). In pathological scar the protein of Skp2 and p27kip1 showed a strong inverse correlation (P < 0.01).
The result indicated that the expression of Skp2 was increased and it may lead to decrease p27kip1 level in the hypertrophic scar and keloid, Skp2 overexpression might play an important role in the proliferation of fibroblasts and in the pathogenesis of pathological scar by adjusting the regulation of cyclins such as p27kip1.
研究S期激酶相关蛋白2（Skp2）基因在病理性瘢痕中的表达及其与p27kip1水平的关系，探讨其在病理性瘢痕发病机制中的作用及可能机制。
采用免疫组织化学技术检测增生性瘢痕（42例）、瘢痕疙瘩（18例）、正常瘢痕（40例）及正常皮肤（50例）中Skp2和p27kip1蛋白的表达及分布情况，并进行统计学分析。
增生性瘢痕与瘢痕疙瘩中Skp2和p27kip1蛋白表达阳性率差异无统计学意义（P>0.05），而正常瘢痕与异常瘢痕比较差异均有统计学意义（P<0.05）。在病理性瘢痕中Skp2与p27kip1蛋白呈显著负相关（P<0.01）。
结果表明增生性瘢痕和瘢痕疙瘩中Skp2表达增加，可能导致p27kip1水平降低，Skp2过表达可能通过调节p27kip1等细胞周期蛋白调控，在成纤维细胞增殖及病理性瘢痕发病机制中起重要作用。
