[原纤维蛋白1在病理性瘢痕中的表达及其意义]

[The expression of fibrillin 1 in pathologic scars and its significance].

作者信息

Nie Fang-Fei, Wang Qi, Qin Ze-Lian

机构信息

Department of Plastic Surgery, Peking University Third Hospital, Beijing 100191, China.

出版信息

Zhonghua Zheng Xing Wai Ke Za Zhi. 2008 Sep;24(5):339-42.

PMID:19119630

Abstract

OBJECTIVE

To probe into the mechanism of fibrillin 1 in pathologic scar, by examining the expressions of fibrillin 1 and TGF-beta1 as well as their correlations in the tissues of keloid, hypertrophic scar and normal skin.

METHODS

The tissues of keloid, hypertrophic scar and normal skin were tested. RT-PCR was used to assess the mRNA expression levels of the aimed genes. The distribution of fibrillin 1 in scars and normal skin was examined by immunohistochemistry staining.

RESULTS

The mRNA level of fibrillin 1 in keloid (0.802 +/- 0.116) was increased by 218.25% (P < 0.01) than that in normal skin (0.252 +/- 0.067). The expression of the gene in hypertrophic scar (0.628 +/- 0.144) was higher by 149.21% (while, P > 0.05) than that in normal skin. The expression of TGF-beta1 in keloid and hypertrophic scar were more than that in normal skin. The expression of fibrillin 1 was related to that of TGF-beta1 positively (r = 0.820, P < 0.01). Fibrillin 1 protein was stained positively in basic membranes, endothelial cells, fibroblasts and extracellular matrix of skin tissues. In dermal, the protein levels of fibrillin 1 in keloid (0.117 +/- 0.042) was decreased than those in normal skin (0.185 +/- 0.043) and hypertrophic scar (0.181 +/- 0.048), the inhibition rates were 36.76%, 35.36% respectively (both P < 0.01).

CONCLUSIONS

The expression of fibrillin 1 in keloid was changed and related to the expression of TGF-beta1 positively, which appears that fibrillin 1 was a cicatrix specific gene. Fibrillin 1 might play an important role in the formation of keloid.

摘要

目的

通过检测瘢痕疙瘩、增生性瘢痕及正常皮肤组织中纤连蛋白1（fibrillin 1）和转化生长因子β1（TGF-β1）的表达情况及其相关性，探讨fibrillin 1在病理性瘢痕形成中的作用机制。

方法

对瘢痕疙瘩、增生性瘢痕及正常皮肤组织进行检测。采用逆转录聚合酶链反应（RT-PCR）检测目的基因的mRNA表达水平，免疫组织化学染色观察fibrillin 1在瘢痕及正常皮肤组织中的分布。

结果

瘢痕疙瘩组织中fibrillin 1的mRNA水平（0.802±0.116）较正常皮肤组织（0.252±0.067）升高了218.25%（P<0.01）。增生性瘢痕组织中该基因的表达水平（0.628±0.144）较正常皮肤组织升高了149.21%（P>0.05）。瘢痕疙瘩和增生性瘢痕组织中TGF-β1的表达均高于正常皮肤组织。fibrillin 1的表达与TGF-β1的表达呈正相关（r=0.820，P<0.01）。免疫组织化学染色显示，fibrillin 1蛋白在皮肤组织的基底膜、内皮细胞、成纤维细胞及细胞外基质中呈阳性表达。在真皮层，瘢痕疙瘩组织中fibrillin 1的蛋白水平（0.117±0.042）低于正常皮肤组织（0.185±0.043）和增生性瘢痕组织（0.181±0.048），抑制率分别为36.76%、35.36%（均P<0.01）。