Hauck Rüdiger, Hafez Hafez M
Institute of Poultry Diseases, Faculty of Veterinary Medicine, Free University Berlin, Berlin, Germany.
J Parasitol. 2010 Apr;96(2):396-400. doi: 10.1645/GE-2267.1.
Phylogenetic trees based on parabasalid sequences of the small subunit rRNA placed Histomonas meleagridis in close proximity to Dientamoeba fragilis, Tritrichomonas foetus, and Monocercomonas sp. In this study, we sequenced partial genes of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), enolase, and alpha-tubulin from 2 strains of H. meleagridis. We found 5 different GAPDH sequences, 6 different enolase sequences, and 3 alpha-tubulin sequences. Phylogenetic trees based on the obtained sequences showed a close relationship of H. meleagridis with T. foetus and, to some extent, Monocercomonas sp. In conclusion, our findings further corroborate the ssu rRNA-based tree. Consequently, our study also supports the hypothesis that H. meleagridis secondarily lost cytoskeletal structures.
基于小亚基核糖体RNA的副基体序列构建的系统发育树显示，火鸡组织滴虫与脆弱双核阿米巴、胎儿三毛滴虫和单鞭滴虫属亲缘关系较近。在本研究中，我们对2株火鸡组织滴虫的甘油醛-3-磷酸脱氢酶（GAPDH）、烯醇化酶和α-微管蛋白的部分基因进行了测序。我们发现了5种不同的GAPDH序列、6种不同的烯醇化酶序列和3种α-微管蛋白序列。基于所得序列构建的系统发育树表明，火鸡组织滴虫与胎儿三毛滴虫以及在一定程度上与单鞭滴虫属关系密切。总之，我们的研究结果进一步证实了基于小亚基核糖体RNA构建的系统发育树。因此，我们的研究也支持火鸡组织滴虫次生失去细胞骨架结构这一假说。
