Yang Huan-Dan, Dong Chen, Guan Feng-Jun, Gao Li-Li, Zhao Tong, Feng Bing-Feng
Department of Pediatrics, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221000, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2010 Jun;12(6):483-7.
To investigate the effect of bone mesenchymal stem cell (BMSC) transplantation on repair of glomerular podocytes and on the Nephrin expression in rats with puromycin aminonucleoside (PAN) -induced nephrosis.
Forty-five Sprague-Dawley rats were randomly divided into three groups (n=15 each): a nephrosis model group that received a single intraperitoneal injection of PAN (0.15 mg/g); a BMSC transplantation group that received a single intraperitoneal injection of PAN (0.15 mg/g) followed by BMSC transfusion; a control group that received a single intraperitoneal injection of normal saline. Ten days after injection, the rats were sacrificed. The 24 hrs urinary protein content and serum albumin and cholesterol levels were measured 24 hrs before sacrifice. Changes of glomerular podocytes were observed under an electron microscope. Brdu labeled positive cells in kidneys were measured by immunohistochemical technology. RT-PCR and Western blot were used to assess the expression of mRNA and protein of Nephrin.
In the nephrosis model group, urinary protein and blood cholesterol contents increased, plasma albumin content decreased compared with those in the control group. Extensive fusion of podocyte foot processes was observed in the nephrosis model group. The BMSC transplantation group had decreased urinary protein and blood cholesterol contents and increased plasma albumin content compared with the nephrosis model group. Fusion of podocyte foot processes was also improved. Brdu labeled positive cells were seen in kidneys in the BMSC transplantation group, but not in the nephrosis model and the control groups. Nephrin mRNA and protein expression decreased significantly in the nephrosis model group compared with that in the control group. The BMSC transplantation group had increased Nephrin mRNA and protein expression compared with the nephrosis model group.
BMSCs can repair glomerular podocytes in PAN-induced nephrosis rats, and the changes of Nephrin expression may be involved in the process.
探讨骨间充质干细胞（BMSC）移植对嘌呤霉素氨基核苷（PAN）诱导的肾病大鼠肾小球足细胞修复及Nephrin表达的影响。
将45只Sprague-Dawley大鼠随机分为三组（每组n = 15）：肾病模型组，单次腹腔注射PAN（0.15 mg/g）；BMSC移植组，单次腹腔注射PAN（0.15 mg/g）后进行BMSC输注；对照组，单次腹腔注射生理盐水。注射10天后处死大鼠。处死前24小时测量24小时尿蛋白含量、血清白蛋白和胆固醇水平。在电子显微镜下观察肾小球足细胞的变化。采用免疫组化技术检测肾脏中Brdu标记的阳性细胞。用RT-PCR和Western blot法评估Nephrin的mRNA和蛋白表达。
与对照组相比，肾病模型组尿蛋白和血胆固醇含量升高，血浆白蛋白含量降低。肾病模型组观察到足细胞足突广泛融合。与肾病模型组相比，BMSC移植组尿蛋白和血胆固醇含量降低，血浆白蛋白含量升高。足细胞足突融合也得到改善。BMSC移植组肾脏中可见Brdu标记的阳性细胞，而肾病模型组和对照组未见。与对照组相比，肾病模型组Nephrin mRNA和蛋白表达明显降低。与肾病模型组相比，BMSC移植组Nephrin mRNA和蛋白表达增加。
BMSCs可修复PAN诱导的肾病大鼠的肾小球足细胞，Nephrin表达的变化可能参与了这一过程。
