间充质干细胞对白化病大鼠抗Thy1,1诱导的肾损伤的影响。
Effect of mesenchymal stem cells on anti-Thy1,1 induced kidney injury in albino rats.
作者信息
Sakr Saber, Rashed Laila, Zarouk Waheba, El-Shamy Rania
机构信息
Department of Zoology, Faculty of Science, Menoufia University, Shebin El-kom, Egypt.
出版信息
Asian Pac J Trop Biomed. 2013 Mar;3(3):174-81. doi: 10.1016/S2221-1691(13)60045-3.
OBJECTIVE
To evaluate the effect of mesenchymal stem cells (MSCs) in rats with anti-Thy1,1 nephritis.
METHODS
Female albino rats were divided into three groups, control group, anti-Thy1,1 group and treatment with i.v. MSCs group. MSCs were derived from bone marrow of male albino rats, Y-chromosome gene was detected by polymerase chain reaction in the kidney. Serum urea and creatinine were estimated for all groups. Kidney of all studied groups was examined histologically and histochemically (total carbohydrates and total proteins). DNA fragmentation and expression of α-SMA were detected.
RESULTS
Kidney of animals injected with anti-Thy1,1 showed inflammatory leucocytic infiltration, hypertrophied glomeruli, tubular necrosis and congestion in the renal blood vessels. The kidney tissue also showed reduction of carbohydrates and total proteins together with increase in apoptosis and in expression of α-SMA. Moreover, the levels of urea and creatinine were elevated. Treating animals with MSCs revealed that kidney tissue displayed an improvement in the histological and histochemical changes. Apoptosis and α-SMA expression were decreased, and the levels of urea and creatinine decreased.
CONCLUSIONS
The obtained results demonstrated the potential of MSCs to ameliorate the structure and function of the kidney in rats with anti-Thy1,1 nephritis possibly through the release of paracrine growth factor(s).
目的
评估间充质干细胞（MSCs）对患抗Thy1,1肾炎大鼠的影响。
方法
将雌性白化大鼠分为三组，即对照组、抗Thy1,1组和静脉注射MSCs治疗组。MSCs取自雄性白化大鼠的骨髓，通过聚合酶链反应检测肾脏中的Y染色体基因。对所有组测定血清尿素和肌酐。对所有研究组的肾脏进行组织学和组织化学检查（总碳水化合物和总蛋白）。检测DNA片段化和α-SMA的表达。
结果
注射抗Thy1,1的动物肾脏显示有炎性白细胞浸润、肾小球肥大、肾小管坏死和肾血管充血。肾脏组织还显示碳水化合物和总蛋白减少，同时细胞凋亡和α-SMA表达增加。此外，尿素和肌酐水平升高。用MSCs治疗动物显示肾脏组织的组织学和组织化学变化有所改善。细胞凋亡和α-SMA表达降低，尿素和肌酐水平下降。
结论
所得结果表明，MSCs可能通过旁分泌生长因子改善患抗Thy1,1肾炎大鼠肾脏的结构和功能。
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