Srivastava Anand, Singh Shailja, Dhawan Shikha, Mahmood Alam M, Mohmmed Asif, Chitnis Chetan E
Malaria Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi 110067, India.
Mol Biochem Parasitol. 2010 Nov;174(1):66-9. doi: 10.1016/j.molbiopara.2010.06.003. Epub 2010 Jun 9.
Plasmodium falciparum belongs to the Apicomplexan group of parasites and is characterised by presence of specialized secretory organelles at the apical end. These apical organelles, referred to as microneme and rhoptries, contain proteins that play important roles during host cell invasion by mediating specific functions such as initial attachment, apical reorientation and junction formation. Recently, a protein referred to as P. falciparum apical sushi protein (PfASP), which is expressed at late schizont stage, was localized to micronemes of P. falciparum merozoites. In the present study, we have used indirect immunofluorescence assays and immunoelectron microscopy to demonstrate that PfASP is localized in the neck of rhoptries and not in micronemes as previously described.
恶性疟原虫属于顶复门寄生虫类群，其特征是在顶端存在特化的分泌细胞器。这些顶端细胞器，即微线体和棒状体，含有在宿主细胞入侵过程中发挥重要作用的蛋白质，它们通过介导诸如初始附着、顶端重新定向和连接形成等特定功能来实现这一点。最近，一种称为恶性疟原虫顶端寿司蛋白（PfASP）的蛋白质，在晚期裂殖体阶段表达，被定位到恶性疟原虫裂殖子的微线体中。在本研究中，我们使用间接免疫荧光测定法和免疫电子显微镜来证明，PfASP定位于棒状体的颈部，而不是如先前所述定位于微线体中。
