烯酰辅酶 A 水合酶短链 1 和过氧化物酶 3 在 PP2 诱导人乳腺癌 MCF-7 细胞凋亡中的作用。
The role of enoyl-CoA hydratase short chain 1 and peroxiredoxin 3 in PP2-induced apoptosis in human breast cancer MCF-7 cells.
机构信息
College of Life Sciences, Peking University, Beijing, PR China.
出版信息
FEBS Lett. 2010 Jul 16;584(14):3185-92. doi: 10.1016/j.febslet.2010.06.002. Epub 2010 Jun 10.
We show that 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2) induces apoptosis and down-regulates the expression of enoyl-CoA hydratase short chain 1 (ECHS1) and peroxiredoxin 3 (PRDX3) in human breast cancer MCF-7 cells. The decrease of ECHS1 and PRDX3 was validated by Western blot and quantitative real-time reverse transcription-PCR in MCF-7 and other carcinoma cells. Knockdown and over-expression of ECHS1 and/or PRDX3 further supported the key role of ECHS1 and PRDX3 in regulation of PP2-induced apoptosis. These results suggest a possible apoptotic pathway whereby down-regulation of ECHS1 and PRDX3 potentiates PP2-induced apoptosis in MCF-7 cells.
我们表明，4-氨基-5-(4-氯苯基)-7-(叔丁基)吡唑并[3,4-d]嘧啶（PP2）可诱导人乳腺癌 MCF-7 细胞凋亡，并下调烯酰辅酶 A 水合酶短链 1（ECHS1）和过氧化物酶 3（PRDX3）的表达。Western blot 和定量实时逆转录-PCR 在 MCF-7 和其他癌细胞中验证了 ECHS1 和 PRDX3 的减少。ECHS1 和/或 PRDX3 的敲低和过表达进一步支持了 ECHS1 和 PRDX3 在调节 PP2 诱导的细胞凋亡中的关键作用。这些结果表明了一种可能的凋亡途径，即 ECHS1 和 PRDX3 的下调增强了 PP2 在 MCF-7 细胞中的诱导凋亡作用。
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