怪手指：WIP 结构域蛋白的功能分析。

Weird fingers: functional analysis of WIP domain proteins.

机构信息

Department of Biology, Chair of Genome Research, Bielefeld University, Bielefeld, Germany.

出版信息

FEBS Lett. 2010 Jul 16;584(14):3116-22. doi: 10.1016/j.febslet.2010.06.007. Epub 2010 Jun 10.

DOI:10.1016/j.febslet.2010.06.007

PMID:20541552

Abstract

WIP proteins form a plant specific subfamily of C2H2 zinc finger (ZF) proteins. In this study, we functionally characterized the WIP domain, which consists of four ZF motifs, and discuss molecular functions for WIP proteins. Mutations in each of the ZFs lead to loss of function of the TT1/WIP1 protein in Arabiopsis thaliana. SV40 type nuclear localisation signals were detected in two of the ZFs and functionally characterized using GFP fusions as well as new mutant alleles identified by TILLING. Promoter swap experiments showed that selected WIP proteins are partially able to take over TT1 function. Activity of the AtBAN promoter, a potential TT1 target, could be increased by the addition of TT1 to the TT2-TT8-TTG1 regulatory complex.

摘要

WIP 蛋白形成了一个植物特有的 C2H2 锌指（ZF）蛋白亚家族。在这项研究中，我们对由四个 ZF 基序组成的 WIP 结构域进行了功能表征，并讨论了 WIP 蛋白的分子功能。在拟南芥中，每个 ZF 的突变都会导致 TT1/WIP1 蛋白的功能丧失。在两个 ZF 中检测到了 SV40 型核定位信号，并通过 GFP 融合以及通过 TILLING 鉴定的新突变等位基因对其进行了功能表征。启动子交换实验表明，所选的 WIP 蛋白在一定程度上能够接管 TT1 的功能。添加 TT1 到 TT2-TT8-TTG1 调节复合物中可以增加 AtBAN 启动子（一个潜在的 TT1 靶标）的活性。