Zhang Shiyang, Liu Junjie, Zhong Guixian, Wang Bo
The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2021 Apr 18;22(8):4197. doi: 10.3390/ijms22084197.
The C2H2-zinc finger protein (C2H2-ZFP) is essential for the regulation of plant development and widely responsive to diverse stresses including drought, cold and salt stress, further affecting the late flavonoid accumulation in higher plants. Tea is known as a popular beverage worldwide and its quality is greatly dependent on the physiological status and growing environment of the tea plant. To date, the understanding of C2H2-ZFP gene family in [L.] O. Kuntze is not yet available. In the present study, 134 Cs genes were identified and randomly distributed on 15 chromosomes. The CsC2H2-ZFP gene family was classified into four clades and gene structures and motif compositions of CsC2H2-ZFPs were similar within the same clade. Segmental duplication and negative selection were the main forces driving the expansion of the CsC2H2-ZFP gene family. Expression patterns suggested that were responsive to different stresses including drought, salt, cold and methyl jasmonate (MeJA) treatment. Specially, several showed a significant correlation with the catechins content and responded to the MeJA treatment, which might contribute to the tea quality and specialized astringent taste. This study will lay the foundations for further research of C2H2-type zinc finger proteins on the stress responses and quality-related metabolites accumulation in .
C2H2型锌指蛋白（C2H2-ZFP）对植物发育调控至关重要，且对包括干旱、寒冷和盐胁迫在内的多种胁迫具有广泛响应，进而影响高等植物中黄酮类化合物的后期积累。茶是全球广受欢迎的饮品，其品质很大程度上取决于茶树的生理状态和生长环境。迄今为止，对[L.] O. Kuntze中C2H2-ZFP基因家族的了解尚不完善。在本研究中，共鉴定出134个Cs基因，它们随机分布在15条染色体上。CsC2H2-ZFP基因家族被分为四个进化枝，同一进化枝内CsC2H2-ZFPs的基因结构和基序组成相似。片段重复和负选择是推动CsC2H2-ZFP基因家族扩张的主要力量。表达模式表明，这些基因对干旱、盐、寒冷和茉莉酸甲酯（MeJA）处理等不同胁迫有响应。特别地，有几个基因与儿茶素含量显著相关，并对MeJA处理有响应，这可能对茶叶品质和特殊的涩味有贡献。本研究将为进一步研究C2H2型锌指蛋白在[具体植物名称未明确]的胁迫响应和品质相关代谢产物积累方面奠定基础。
