Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
J Ethnopharmacol. 2010 Aug 19;131(1):22-7. doi: 10.1016/j.jep.2010.05.043. Epub 2010 Jun 11.
Leucosidea sericea is used as a vermifuge and in the treatment of ophthalmia by various tribes in southern African countries.
The study aimed at screening leaves and stems of Leucosidea sericea for pharmacological activity and validating the plant's traditional use. A general phytochemical screening was also carried out.
Petroleum ether (PE), dichloromethane (DCM), ethanol (EtOH) and water extracts of the plant parts were investigated for antimicrobial, anthelmintic and cyclooxygenase (COX) inhibitory activities. Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) and Candida albicans were used for the antimicrobial evaluation. Caenorhabditis elegans was used for the anthelmintic assay using the microdilution technique. Cyclooxygenase-1 and -2 (COX-1 and -2) were used to evaluate the anti-inflammatory potential of the plant extracts. Phytochemical analysis for phenolic compounds, including gallotannins, condensed tannins and flavonoids was done using 50% methanol extracts of the leaves and stems employing spectrophotometric methods.
The leaf extracts exhibited broad spectrum antibacterial activity ranging from 0.025 to 6.25mg/ml. The most noteworthy minimum inhibitory concentration (MIC) of 0.025 mg/ml was exhibited by PE and DCM leaf extracts against Bacillus subtilis and Staphylococcus aureus, respectively. In the anthelmintic assay, the best minimum lethal concentration (MLC) value of 0.26 mg/ml was observed for the DCM and EtOH leaf extracts. Both leaf and stem organic solvent extracts exhibited high to moderate inhibition against COX-1 and -2 at a screening concentration of 250 microg/ml. At lower concentrations, the extracts displayed a dose-dependent inhibition, with the lowest IC(50) values of 0.06 microg/ml (COX-1) and 12.66 microg/ml (COX-2) exhibited by the PE extract of the leaves. Generally, the leaf extracts exhibited better pharmacological activities and contained higher amounts of phenolic compounds than the stem extracts. Alkaloids and saponins were only detected in the leaf and stem extracts, respectively.
The reported results support the local use of Leucosidea sericea against eye infections and as a vermifuge. The pharmacological activities exhibited by the leaf extracts are probably due to their higher phenolic levels.
在南非国家的不同部落中，Leucosidea sericea 被用作驱虫剂和治疗眼炎的药物。
本研究旨在筛选 Leucosidea sericea 的叶和茎，以验证其药理学活性，并验证该植物的传统用途。还进行了一般的植物化学筛选。
研究了植物部分的石油醚（PE）、二氯甲烷（DCM）、乙醇（EtOH）和水提取物的抗菌、驱虫和环氧化酶（COX）抑制活性。使用革兰氏阳性菌（枯草芽孢杆菌和金黄色葡萄球菌）、革兰氏阴性菌（大肠杆菌和肺炎克雷伯菌）和白色念珠菌进行抗菌评价。使用微量稀释技术，使用秀丽隐杆线虫进行驱虫测定。使用 COX-1 和 COX-2 评估植物提取物的抗炎潜力。使用分光光度法，用 50%甲醇提取物对叶和茎进行酚类化合物（包括没食子鞣质、缩合单宁和类黄酮）的植物化学分析。
叶提取物表现出广谱的抗菌活性，范围从 0.025 到 6.25mg/ml。PE 和 DCM 叶提取物对枯草芽孢杆菌和金黄色葡萄球菌的最低抑菌浓度（MIC）分别为 0.025mg/ml，最为显著。在驱虫测定中，DCM 和 EtOH 叶提取物的最低致死浓度（MLC）值为 0.26mg/ml。有机溶剂提取物的叶和茎均对 COX-1 和 COX-2 表现出高至中度抑制，在筛选浓度为 250μg/ml 时。在较低浓度下，提取物表现出剂量依赖性抑制，PE 叶提取物的最低 IC50 值分别为 0.06μg/ml（COX-1）和 12.66μg/ml（COX-2）。通常，叶提取物表现出更好的药理活性，并且比茎提取物含有更高量的酚类化合物。仅在叶和茎提取物中检测到生物碱和皂苷。
报告的结果支持 Leucosidea sericea 用于眼部感染和驱虫的地方用途。叶提取物表现出的药理活性可能归因于其较高的酚类水平。
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