Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
J Ethnopharmacol. 2011 Apr 12;134(3):775-80. doi: 10.1016/j.jep.2011.01.039. Epub 2011 Feb 1.
The study aimed to compare the antimicrobial and phytochemical properties of in vitro cultured and outdoor grown Tulbaghia violacea plants in the quest to validate the use of micropropagated plants as alternatives to outdoor grown plants in traditional medicine. Tulbaghia violacea is used extensively in South African traditional medicine for HIV/AIDS patients and in the treatment of gastrointestinal ailments, asthma, fever and tuberculosis.
Extracts of micropropagated and outdoor grown Tulbaghia violacea plants were evaluated for their antibacterial and antifungal activities against Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and a fungus Candida albicans using microdilution methods. Saponins and phenolic compounds including condensed tannins, gallotannins and flavonoids were quantitatively determined using spectrophotometric methods. A qualitative test for saponins was also carried out.
The petroleum ether (PE) extracts of micropropagated plants and dichloromethane (DCM) extracts of outdoor grown plants showed good antibacterial activity, each against two bacterial test strains. PE extracts of micropropagated plants showed the best antibacterial activity with a minimum inhibitory concentration (MIC) of 0.39 mg/ml against Bacillus subtilis. Good MIC (<1mg/ml) and minimum fungicidal concentration (MFC) values of 0.78 mg/ml were only obtained in DCM extracts of outdoor grown plants. MIC and MFC values for water and ethanol extracts of both micropropagated and outdoor grown plants were similar and in the range 3.125-12.5mg/ml. Total phenolics, gallotannins, flavonoids and saponins were significantly higher in micropropagated plants than in outdoor grown ones. In all cases, the amounts of phytochemical compounds in micropropagated plants were more than twice that of outdoor grown plants except for condensed tannins.
The results form a good basis for the use of Tulbaghia violacea micropropagated plants as a complement to the outdoor grown plants in traditional medicine.
本研究旨在比较体外培养和户外生长的 Tulbaghia violacea 植物的抗菌和植物化学特性，以验证在传统医学中使用微繁殖植物替代户外生长植物的合理性。Tulbaghia violacea 在南非传统医学中被广泛用于治疗 HIV/AIDS 患者和胃肠道疾病、哮喘、发烧和肺结核。
使用微量稀释法评估微繁殖和户外生长的 Tulbaghia violacea 植物的提取物对枯草芽孢杆菌、大肠杆菌、肺炎克雷伯菌、金黄色葡萄球菌和真菌白色念珠菌的抗菌和抗真菌活性。使用分光光度法定量测定皂苷和酚类化合物，包括缩合单宁、没食子单宁和类黄酮。还进行了皂苷的定性测试。
微繁殖植物的石油醚（PE）提取物和户外生长植物的二氯甲烷（DCM）提取物表现出良好的抗菌活性，每种提取物都能抑制两种细菌测试菌株。微繁殖植物的 PE 提取物对枯草芽孢杆菌的最小抑菌浓度（MIC）为 0.39mg/ml，表现出最佳的抗菌活性。仅在户外生长植物的 DCM 提取物中获得了良好的 MIC（<1mg/ml）和最小杀菌浓度（MFC）值 0.78mg/ml。微繁殖和户外生长植物的水和乙醇提取物的 MIC 和 MFC 值相似，范围为 3.125-12.5mg/ml。微繁殖植物的总酚类、没食子单宁、类黄酮和皂苷含量明显高于户外生长植物。在所有情况下，除了缩合单宁外，微繁殖植物中的植物化学化合物含量均是户外生长植物的两倍以上。
这些结果为在传统医学中使用 Tulbaghia violacea 微繁殖植物作为户外生长植物的补充提供了良好的基础。
