Enteric Viruses Group, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411 001, Maharashtra, India.
Infect Genet Evol. 2010 Oct;10(7):940-9. doi: 10.1016/j.meegid.2010.05.017. Epub 2010 Jun 11.
NSP4 and VP6 genes of a total of 118 rotavirus strains detected in adolescent and adult cases of acute gastroenteritis (AGE) in 1993-1996 and 2004-2007 were characterized to determine their diversity and genetic linkage. Eighty-two percent and 89% of the strains showed amplification of NSP4 and VP6 genes respectively in RT-PCR. Sequencing and phylogenetic analysis of the VP6 genes showed distribution of genogroups in the lineages I-1 (1.4%), I-2 (50.7%) and II-4 (47.9%) in the 1990s and I-2 (73.5%) and II-4 (26.5%) in 2000s, indicating diversity in genogroups at both time points. Amino acid divergence within the genogroup II strains from 1990s and genogroup I strains from the 2000s was noteworthy (4.7-6.7%). Sequencing and phylogenetic analysis of the NSP4 genes showed almost equal distribution (45.0-55.0%) of genotypes A and B however, higher amino acid divergence within the genotype B strains (up to 9.3%) than in genotype A strains (up to 2.9%) at the two-time points. Nearly 70% of the strains showed NSP4-A-VP6-I or NSP4-B-VP6-II genetic linkage. The discordance in the linkage noted in 29.7% of the strains was predominated by NSP4-B and VP6-I combination and appeared strikingly high in the infections caused by unusual and mixed rotavirus strains. This is the first report to describe the phylogenetic analysis of rotavirus NSP4 and VP6 genes and their discordance in adolescent and adult cases with AGE from India. The extensive diversity within the rotavirus genes and their relationship revealed by this study emphasizes the need for evaluation of the rotavirus vaccines being used currently.
对 1993-1996 年和 2004-2007 年期间检测到的 118 株青少年和成人急性肠胃炎（AGE）病例中 NSP4 和 VP6 基因的特征进行了研究，以确定它们的多样性和遗传联系。82%和 89%的毒株在 RT-PCR 中分别扩增出 NSP4 和 VP6 基因。VP6 基因的测序和系统发育分析显示，90 年代的 I-1 组（1.4%）、I-2 组（50.7%）和 II-4 组（47.9%）和 2000 年代的 I-2 组（73.5%）和 II-4 组（26.5%）。表明两个时间点的基因群多样性。90 年代的 II 组和 2000 年代的 I 组毒株之间的氨基酸差异值得注意（4.7-6.7%）。NSP4 基因的测序和系统发育分析显示，A 型和 B 型基因型的分布几乎相等（45.0-55.0%），但在两个时间点，B 型基因型的菌株内氨基酸差异较大（高达 9.3%）比 A 型菌株（高达 2.9%）。近 70%的菌株显示 NSP4-A-VP6-I 或 NSP4-B-VP6-II 遗传联系。在 29.7%的菌株中观察到的这种不匹配主要是由于 NSP4-B 和 VP6-I 的组合，并且在由不寻常和混合轮状病毒株引起的感染中，这种不匹配的情况非常高。这是首次描述印度青少年和成人 AGE 病例中轮状病毒 NSP4 和 VP6 基因的系统发育分析及其不匹配情况的报告。本研究揭示了轮状病毒基因的广泛多样性及其关系，强调了需要评估目前使用的轮状病毒疫苗。
