Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA.
Vet J. 2011 May;188(2):221-7. doi: 10.1016/j.tvjl.2010.05.015. Epub 2010 Jun 12.
Leukotoxin (LKT) is a virulence factor for Mannheimia haemolytica. In this study, bovine alveolar macrophages (BAMs) were challenged with wild type (wt) and LKT deficient (lkt(-)) M. haemolytica at a concentration of 1 bacterium/BAM and the cytokine response was quantified by ELISA and real-time reverse transcriptase-PCR. Significant increases in protein concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-10 were observed in supernatants obtained from BAMs challenged with the lkt(-) strain of M. haemolytica compared with wt challenged BAMs. There were no significant differences in mRNA expression of TNFα, IL-1ß, IL-6, IL-8 or IL-10 between BAMs challenged with the lkt(-) strain of M. haemolytica compared with wt challenged BAMs. BAMs challenged with the wt strain exhibited, on average, 43% more cytotoxicity than lkt(-) challenged BAMs (P<0.01).
白细胞毒素 (LKT) 是溶血曼海姆菌的一种毒力因子。在这项研究中，牛肺泡巨噬细胞 (BAMs) 分别用野生型 (wt) 和白细胞毒素缺陷型 (lkt(-)) 溶血曼海姆菌以 1 个细菌/BAM 的浓度进行刺激，通过 ELISA 和实时逆转录聚合酶链式反应定量细胞因子反应。与 wt 刺激的 BAMs 相比，用 lkt(-) 溶血曼海姆菌刺激的 BAMs 的上清液中肿瘤坏死因子 (TNF)-α 和白细胞介素 (IL)-10 的蛋白浓度显著增加。与 wt 刺激的 BAMs 相比，用 lkt(-) 溶血曼海姆菌刺激的 BAMs 中 TNFα、IL-1ß、IL-6、IL-8 或 IL-10 的 mRNA 表达没有显著差异。用 wt 菌株刺激的 BAMs 的细胞毒性比 lkt(-) 菌株刺激的 BAMs 平均高 43%（P<0.01）。
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