Xer 位点特异性重组，一种向分枝杆菌中引入无标记缺失的有效工具。
Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.
机构信息
Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, Via Gabelli 63, 35121 Padova, Italy.
出版信息
Appl Environ Microbiol. 2010 Aug;76(15):5312-6. doi: 10.1128/AEM.00382-10. Epub 2010 Jun 11.
Abstract
Genetic manipulation of mycobacteria still represents a serious challenge due to the lack of tools and selection markers. In this report, we describe the development of an intrinsically unstable excisable cassette for introduction of unmarked mutations in both Mycobacterium smegmatis and Mycobacterium tuberculosis.
摘要
由于缺乏工具和选择标记，分枝杆菌的遗传操作仍然是一个严峻的挑战。在本报告中，我们描述了一种内在不稳定的可切除盒的开发，用于在耻垢分枝杆菌和结核分枝杆菌中引入无标记突变。
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