高效生成无标记缺失的嗜肺军团菌。
Efficient generation of unmarked deletions in Legionella pneumophila.
机构信息
Department of Microbiology and Immunology, University of Michigan Medical School, 5641 Medical Sciences Building II, 1150 West Medical Center Dr., Ann Arbor, MI 48109-5620, USA.
出版信息
Appl Environ Microbiol. 2011 Apr;77(7):2545-8. doi: 10.1128/AEM.02904-10. Epub 2011 Feb 4.
Abstract
Unmarked gene deletions facilitate studies of Legionella pneumophila multicomponent processes, such as motility and exonuclease activity. For this purpose, FRT-flanked alleles constructed in Escherichia coli using λ-Red recombinase were transferred to L. pneumophila by natural transformation. Resistance cassettes were then efficiently excised using the Flp site-specific recombinase encoded on a plasmid that is readily lost.
摘要
未标记的基因缺失有助于研究嗜肺军团菌的多组分过程，例如运动性和核酸外切酶活性。为此，使用 λ-Red 重组酶在大肠杆菌中构建的 FRT 侧翼等位基因通过自然转化转移到嗜肺军团菌中。然后使用易于丢失的质粒上编码的 Flp 位点特异性重组酶有效地切除抗性盒。
相似文献
1
Efficient generation of unmarked deletions in Legionella pneumophila.高效生成无标记缺失的嗜肺军团菌。
Appl Environ Microbiol. 2011 Apr;77(7):2545-8. doi: 10.1128/AEM.02904-10. Epub 2011 Feb 4.
2
Constructing unmarked gene deletions in Legionella pneumophila.构建嗜肺军团菌中无标记基因缺失体
Methods Mol Biol. 2013;954:197-212. doi: 10.1007/978-1-62703-161-5_10.
3
Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration.新型高效分离梭菌双交叉等位基因交换突变体的系统，实现了无标记染色体基因缺失和 DNA 整合。
Appl Environ Microbiol. 2012 Nov;78(22):8112-21. doi: 10.1128/AEM.02214-12. Epub 2012 Sep 14.
4
A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination.通过 FLP/FRT 重组将无标记突变引入非感受态革兰氏阴性菌大基因的一种新的简单方法。
BMC Microbiol. 2013 Apr 17;13:86. doi: 10.1186/1471-2180-13-86.
5
Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.Xer 位点特异性重组，一种向分枝杆菌中引入无标记缺失的有效工具。
Appl Environ Microbiol. 2010 Aug;76(15):5312-6. doi: 10.1128/AEM.00382-10. Epub 2010 Jun 11.
6
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant.大肠杆菌中的基因破坏：带有Flp催化切除抗生素抗性决定簇选项的TcR和KmR盒式结构。
Gene. 1995 May 26;158(1):9-14. doi: 10.1016/0378-1119(95)00193-a.
7
Scar-Free Genome Editing in Legionella pneumophila.嗜肺军团菌中的无疤痕基因组编辑
Methods Mol Biol. 2019;1921:93-105. doi: 10.1007/978-1-4939-9048-1_6.
8
Escherichia coli F plasmid transfers to and replicates within Legionella pneumophila: an alternative to using an RP4-based system for gene delivery.大肠杆菌F质粒转移至嗜肺军团菌并在其中复制：一种替代基于RP4系统进行基因传递的方法。
Plasmid. 1994 Nov;32(3):280-94. doi: 10.1006/plas.1994.1067.
9
Efficient transformation of Legionella pneumophila by high-voltage electroporation.通过高压电穿孔高效转化嗜肺军团菌。
Microbiol Res. 2006;161(3):246-51. doi: 10.1016/j.micres.2005.09.001. Epub 2005 Nov 11.
10
Characterization of the cryptic plasmid pOfk55 from Legionella pneumophila and construction of a pOfk55-derived shuttle vector.嗜肺军团菌隐蔽质粒pOfk55的特性分析及基于pOfk55的穿梭载体构建
Plasmid. 2017 Mar;90:30-37. doi: 10.1016/j.plasmid.2017.02.004. Epub 2017 Mar 1.
引用本文的文献
1
The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans.军团菌胶原蛋白样蛋白采用独特的结合机制识别宿主糖胺聚糖。
Nat Commun. 2024 Jun 8;15(1):4912. doi: 10.1038/s41467-024-49255-4.
2
Legionella pneumophila Rhizoferrin Promotes Bacterial Biofilm Formation and Growth within Amoebae and Macrophages.嗜肺军团菌根铁蛋白促进细菌生物膜在变形虫和巨噬细胞内的形成和生长。
Infect Immun. 2023 Aug 16;91(8):e0007223. doi: 10.1128/iai.00072-23. Epub 2023 Jul 10.
3
Legionella pneumophila Cas2 Promotes the Expression of Small Heat Shock Protein C2 That Is Required for Thermal Tolerance and Optimal Intracellular Infection.嗜肺军团菌 Cas2 促进小热休克蛋白 C2 的表达，该蛋白对于热耐受和最佳细胞内感染是必需的。
Infect Immun. 2022 Oct 20;90(10):e0036922. doi: 10.1128/iai.00369-22. Epub 2022 Sep 8.
4
Stenotrophomonas maltophilia Encodes a VirB/VirD4 Type IV Secretion System That Modulates Apoptosis in Human Cells and Promotes Competition against Heterologous Bacteria, Including Pseudomonas aeruginosa.嗜麦芽寡养单胞菌编码一种 VirB/VirD4 型 IV 型分泌系统，该系统可调节人细胞中的细胞凋亡，并促进与包括铜绿假单胞菌在内的异源细菌的竞争。
Infect Immun. 2019 Aug 21;87(9). doi: 10.1128/IAI.00457-19. Print 2019 Sep.
5
A Two-Component System That Modulates Cyclic di-GMP Metabolism Promotes Legionella pneumophila Differentiation and Viability in Low-Nutrient Conditions.一种调节环二鸟苷酸代谢的双组分系统促进军团菌在低营养条件下的分化和存活。
J Bacteriol. 2019 Aug 8;201(17). doi: 10.1128/JB.00253-19. Print 2019 Sep 1.
6
The Toolbox for Uncovering the Functions of Dot/Icm Type IVb Secretion System Effectors: Current State and Future Directions.揭示 Dot/Icm 型 IVb 分泌系统效应子功能的工具箱：现状与未来方向。
Front Cell Infect Microbiol. 2018 Jan 5;7:528. doi: 10.3389/fcimb.2017.00528. eCollection 2017.
7
Stenotrophomonas maltophilia produces an EntC-dependent catecholate siderophore that is distinct from enterobactin.嗜麦芽窄食单胞菌产生一种依赖EntC的儿茶酚型铁载体，该铁载体与肠杆菌素不同。
Microbiology (Reading). 2017 Nov;163(11):1590-1603. doi: 10.1099/mic.0.000545. Epub 2017 Oct 6.
8
Stenotrophomonas maltophilia Serine Protease StmPr1 Induces Matrilysis, Anoikis, and Protease-Activated Receptor 2 Activation in Human Lung Epithelial Cells.嗜麦芽窄食单胞菌丝氨酸蛋白酶StmPr1诱导人肺上皮细胞发生基质溶解、失巢凋亡及蛋白酶激活受体2激活。
Infect Immun. 2017 Nov 17;85(12). doi: 10.1128/IAI.00544-17. Print 2017 Dec.
9
Type II Secretion Is Necessary for Optimal Association of the Legionella-Containing Vacuole with Macrophage Rab1B but Enhances Intracellular Replication Mainly by Rab1B-Independent Mechanisms.II型分泌对于含军团菌液泡与巨噬细胞Rab1B的最佳结合是必需的，但主要通过Rab1B非依赖机制增强细胞内复制。
Infect Immun. 2016 Nov 18;84(12):3313-3327. doi: 10.1128/IAI.00750-16. Print 2016 Dec.
10
csrT Represents a New Class of csrA-Like Regulatory Genes Associated with Integrative Conjugative Elements of Legionella pneumophila.csrT代表一类与嗜肺军团菌整合性接合元件相关的新型类csrA调控基因。
J Bacteriol. 2015 Nov 23;198(3):553-64. doi: 10.1128/JB.00732-15. Print 2016 Feb 1.
本文引用的文献
1
Recombineering: genetic engineering in bacteria using homologous recombination.重组工程：利用同源重组在细菌中进行基因工程。
Curr Protoc Mol Biol. 2014 Apr 14;106:1.16.1-1.16.39. doi: 10.1002/0471142727.mb0116s106.
2
Oligonucleotides stimulate genomic alterations of Legionella pneumophila.寡核苷酸刺激嗜肺军团菌基因组改变。
Mol Microbiol. 2011 Apr;80(1):231-47. doi: 10.1111/j.1365-2958.2011.07573.x. Epub 2011 Feb 24.
3
Modulation of host cell function by Legionella pneumophila type IV effectors.军团菌属Ⅳ型效应物对宿主细胞功能的调节。
Annu Rev Cell Dev Biol. 2010;26:261-83. doi: 10.1146/annurev-cellbio-100109-104034.
4
Molecular pathogenesis of infections caused by Legionella pneumophila.嗜肺军团菌感染的分子发病机制。
Clin Microbiol Rev. 2010 Apr;23(2):274-98. doi: 10.1128/CMR.00052-09.
5
Many substrates and functions of type II secretion: lessons learned from Legionella pneumophila.II型分泌的多种底物和功能：从嗜肺军团菌中获得的经验教训。
Future Microbiol. 2009 Sep;4(7):797-805. doi: 10.2217/fmb.09.53.
6
The Legionella pneumophila replication vacuole: making a cosy niche inside host cells.嗜肺军团菌复制泡：在宿主细胞内营造一个舒适的小环境。
Nat Rev Microbiol. 2009 Jan;7(1):13-24. doi: 10.1038/nrmicro1967. Epub 2008 Nov 17.
7
Multicopy plasmid modification with phage lambda Red recombineering.利用噬菌体λ Red重组工程进行多拷贝质粒修饰
Plasmid. 2007 Sep;58(2):148-58. doi: 10.1016/j.plasmid.2007.03.001. Epub 2007 Apr 16.
8
RecA-independent recombination is efficient but limited by exonucleases.不依赖RecA的重组效率高，但受到核酸外切酶的限制。
Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):216-21. doi: 10.1073/pnas.0608293104. Epub 2006 Dec 20.
9
Efficient transformation of Legionella pneumophila by high-voltage electroporation.通过高压电穿孔高效转化嗜肺军团菌。
Microbiol Res. 2006;161(3):246-51. doi: 10.1016/j.micres.2005.09.001. Epub 2005 Nov 11.
10
Components of the Legionella pneumophila flagellar regulon contribute to multiple virulence traits, including lysosome avoidance and macrophage death.嗜肺军团菌鞭毛调节子的组成部分有助于多种毒力特性，包括避免溶酶体作用和巨噬细胞死亡。
Infect Immun. 2005 Sep;73(9):5720-34. doi: 10.1128/IAI.73.9.5720-5734.2005.
Zotero 插件