Kudo K, Nagata T, Imamura T, Kage S, Hida Y
Department of Forensic Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Int J Legal Med. 1991 Mar;104(2):67-9. doi: 10.1007/BF01626033.
A reliable and sensitive method has been developed to assess the concentrations of the hypnotic drug triazolam in human tissues, including putrefied tissues. The method involves a 3-step solvent extraction, clean-up on a silica gel column and gas chromatography using a nitrogen phosphorus detector and a capillary column. Estazolam was used as an internal standard. The calibration curve was linear over the concentration range 1 ng/g-1 microgram/g and the lower limit of detection was 0.5 ng/g. A forensic study was performed on the toxicological effects of triazolam using putrefied tissues.
已开发出一种可靠且灵敏的方法来评估人体组织（包括腐败组织）中催眠药物三唑仑的浓度。该方法包括三步溶剂萃取、硅胶柱净化以及使用氮磷检测器和毛细管柱的气相色谱法。艾司唑仑用作内标。校准曲线在1 ng/g至1微克/克的浓度范围内呈线性，检测下限为0.5 ng/g。使用腐败组织对三唑仑的毒理学效应进行了法医研究。
