死后样本中三唑并苯二氮䓬类药物三唑仑的测定。

The determination of the triazolobenzodiazepine triazolam in post mortem samples.

作者信息

Wong R J

出版信息

J Anal Toxicol. 1984 Jan-Feb;8(1):10-3. doi: 10.1093/jat/8.1.10.

DOI:10.1093/jat/8.1.10

Abstract

A post mortem case involving the triazolobenzodiazepine triazolam is reported. Levels of 38 ng/g in the liver and 6.7 ng/mL in the blood were determined by reversed-phase high-performance liquid chromatography (HPLC) using an eluent of 0.06% acetic acid:acetonitrile (61:39) and ultraviolet detection at 221 nm. The presence of triazolam in the post mortem samples was verified by its oxidation to a benzophenone with potassium dichromate, followed by the analysis of the oxidized extract by HPLC using a silica column and an eluent of dichloromethane:methanol (97.5:2.5) at 250 nm.

摘要

报告了一例涉及三唑并苯二氮䓬类药物三唑仑的尸检案例。采用0.06%乙酸:乙腈（61:39）作为洗脱液，在221nm处进行紫外检测，通过反相高效液相色谱法（HPLC）测定肝脏中三唑仑含量为38 ng/g，血液中为6.7 ng/mL。通过用重铬酸钾将尸检样品中的三唑仑氧化为二苯甲酮，随后使用硅胶柱，以二氯甲烷:甲醇（97.5:2.5）作为洗脱液，在250nm处通过HPLC对氧化提取物进行分析，验证了尸检样品中三唑仑的存在。