Department of Microbiology, University Hospital of Antwerp, Wilrijkstraat 10, Edegem, Belgium.
Vet Rec. 2010 Jun 12;166(24):753-6. doi: 10.1136/vr.b4862.
Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. Information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. Twenty-six (74 per cent) of the serum samples were positive in the rose bengal test and 29 (83 per cent) were positive by indirect ELISA. Three isolates of Brucella, biotyped as Brucella abortus biovar 3, were cultured from six hygroma fluid samples. The multiple locus variable number tandem repeat analysis assay clustered the isolates as B abortus with the same profile for the three isolates, suggesting a common origin of contamination.
从冈比亚西部地区一个有临床布鲁氏菌病（流产和黏液囊炎）症状的性成熟牛群中采集了 35 份血清样本和 6 份黏液囊液样本，以便分离和鉴定布鲁氏菌属。还为每个采样动物收集了有关其性别、年龄、产犊次数、流产次数、黏液囊炎存在情况和睾丸炎存在情况的信息。26 份（74%）血清样本在玫瑰红孟加拉试验中呈阳性，29 份（83%）间接 ELISA 呈阳性。从 6 份黏液囊液样本中培养出了 3 株布鲁氏菌，生物型为布鲁氏菌流产亚种 3。多位点可变数串联重复分析检测将这些分离株聚类为 B. abortus，这 3 株分离株具有相同的图谱，表明存在共同的污染来源。
