衰减全反射傅里叶变换红外光谱法对聚合物表面蛋白质吸附的分析

ATR-FTIR analysis of protein adsorption on polymeric surfaces.

作者信息

van Straaten J, Peppas N A

机构信息

School of Chemical Engineering, Purdue University, West Lafayette, IN 47907.

出版信息

J Biomater Sci Polym Ed. 1991;2(2):113-21. doi: 10.1163/156856291x00106.

DOI:10.1163/156856291x00106

PMID:2054330

Abstract

ATR-FTIR spectroscopy was used to study the adsorption of bovine serum albumin on poly(vinyl chloride), poly(vinylidene fluoride), poly(methyl methacrylate), and poly(ethylene glycol)-grafted poly(methyl methacrylate) surfaces. The results were analyzed in terms of the amount of albumin adsorbed on the various polymeric surfaces when immersed in a 1 g/1 protein solution. Experimental results were compared with the Van Straaten and Peppas model (1990) and satisfactory agreement was obtained. It was confirmed that poly(ethylene glycol) surfaces exhibited the lowest albumin adsorption patterns.

摘要

衰减全反射傅里叶变换红外光谱法（ATR-FTIR）用于研究牛血清白蛋白在聚氯乙烯、聚偏二氟乙烯、聚甲基丙烯酸甲酯以及聚乙二醇接枝的聚甲基丙烯酸甲酯表面的吸附情况。根据将各种聚合物表面浸入1 g/1蛋白质溶液中时吸附的白蛋白量对结果进行了分析。将实验结果与范·斯特拉滕和佩帕斯模型（1990年）进行了比较，并获得了满意的一致性。证实聚乙二醇表面表现出最低的白蛋白吸附模式。