Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513, Teltow, Germany; Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14476 Potsdam, Germany; Helmholtz Virtual Institute - Multifunctional Biomaterials for Medicine, Kantstr. 55, 14513, Teltow, Germany.
Macromol Biosci. 2013 Dec;13(12):1720-9. doi: 10.1002/mabi.201300309. Epub 2013 Oct 29.
The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) membranes by grafting with monoamino PEG of different chain lengths (Mn =1 kDa or 10 kDa) and end groups (methoxy or hydroxyl) is proven by spectroscopy, changes of surface hydrophilicity, and surface shielding effects. The surface functionalization does lead to reduction of adsorption of BSA, but not of fibrinogen. The thrombocyte adhesion is increased compared to untreated PEI surfaces. Conclusively, rough instead of smooth polymer or gold surfaces should be investigated as relevant models.
线性 PEG 接枝在光滑表面上的链长和端基已知会影响蛋白质吸附和血小板黏附。在这里，我们探索了已建立的结构-功能关系是否可以转移到相关的粗糙表面。通过接枝不同链长（Mn = 1 kDa 或 10 kDa）和端基（甲氧基或羟基）的单氨基聚乙二醇，对聚醚酰亚胺（PEI）膜进行功能化，通过光谱法、表面亲水性变化和表面屏蔽效应证明了这一点。表面功能化确实导致 BSA 的吸附减少，但纤维蛋白原的吸附没有减少。与未经处理的 PEI 表面相比，血小板黏附增加。结论是，应该研究粗糙而不是光滑的聚合物或金表面作为相关模型。
