Schaumburg-Lever G, Metzler G, Tronnier M
Department of Dermatology, Eberhard-Karls-Universität, Tübingen, F.R.G.
J Dermatol Sci. 1991 Jan;2(1):55-61. doi: 10.1016/0923-1811(91)90043-w.
The distribution of carbohydrate residues in eccrine and apocrine glands of normal human skin was studied using a post-embedding technique with Lowicryl K4M. Thin sections were incubated with Ulex europaeus agglutinin I (UEA I), wheat germ agglutinin (WGA), peanut agglutinin (PNA), concanavalin A (Con A), soybean agglutinin (SBA), and dolichos biflorus agglutinin (DBA). All lectins except for PNA showed labeling of the plasma membranes of dark cells, clear cells, and apocrine cells. The granules of the eccrine gland were labeled with all lectins except for DBA. The mitochondrial granules of the apocrine gland were not labeled with any lectin, whereas the lysosomal granules showed a positive reaction with all lectins except for PNA. After incubation with PNA, in eccrine glands the granules were the only structure labeled, whereas in apocrine glands the luminal side of the plasma membrane and cytoplasmic vesicles beneath it were the only structures labeled.
采用Lowicryl K4M包埋后技术，研究了正常人体皮肤中汗腺和顶泌汗腺碳水化合物残基的分布。将薄切片与欧洲荆豆凝集素I（UEA I）、麦胚凝集素（WGA）、花生凝集素（PNA）、伴刀豆球蛋白A（Con A）、大豆凝集素（SBA）和双花扁豆凝集素（DBA）一起孵育。除PNA外，所有凝集素均显示对暗细胞、明细胞和顶泌细胞的质膜有标记。汗腺的颗粒除DBA外均被所有凝集素标记。顶泌汗腺的线粒体颗粒未被任何凝集素标记，而溶酶体颗粒除PNA外与所有凝集素均呈阳性反应。用PNA孵育后，在汗腺中颗粒是唯一被标记的结构，而在顶泌汗腺中质膜的腔面及其下方的细胞质囊泡是唯一被标记的结构。
