Tsubura A, Fujita Y, Sasaki M, Morii S
Department of Pathology, Kansai Medical University, Osaka, Japan.
J Cutan Pathol. 1992 Dec;19(6):483-9. doi: 10.1111/j.1600-0560.1992.tb01601.x.
A histochemical investigation of lectin-binding sites was carried out on formalin-fixed paraffin-embedded sections of 60 skin appendage tumors and adjacent normal skin appendages, using four different biotinylated lectins, peanut agglutinin (PNA), Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA), and Ulex europaeus agglutinin-1 (UEA-1), and avidin-biotin-horseradish peroxidase labeling. In the secretory segments of eccrine sweat glands, the superficial dark cells showed strong cytoplasmic staining with UEA-1, whereas DBA and SBA strongly stained the plasma membranes of basal clear cells. The acinar cells of apocrine sweat glands revealed sporadic apical membrane staining with all four lectins. In some cases, the luminal membranes of sweat gland ducts showed apical membranous staining with all four lectins. In the hair follicles, the inner root sheath was positive for all four lectins, and the outer root sheath was stained by PNA. The sebaceous ducts, as well as the outer root sheath at the level of sebaceous duct insertion, were also labeled by all four lectins. Sebaceous lobules showed cytoplasmic and membrane staining of mature sebocytes with PNA and SBA. Although sweat gland tumors revealed differences in lectin binding when compared to their corresponding normal tissues, the lectin-binding pattern of pilosebaceous tumors was analogous to the pilosebaceous apparatus.
利用四种不同的生物素化凝集素，即花生凝集素（PNA）、双花扁豆凝集素（DBA）、大豆凝集素（SBA）和欧洲荆豆凝集素-1（UEA-1），以及抗生物素蛋白-生物素-辣根过氧化物酶标记法，对60例皮肤附属器肿瘤及相邻正常皮肤附属器的福尔马林固定石蜡包埋切片进行凝集素结合位点的组织化学研究。在小汗腺的分泌段，浅暗细胞显示出UEA-1强细胞质染色，而DBA和SBA强烈染色基底透明细胞的质膜。大汗腺的腺泡细胞在所有四种凝集素作用下均显示散在的顶端膜染色。在某些情况下，汗腺导管的腔膜在所有四种凝集素作用下均显示顶端膜染色。在毛囊中，内根鞘对所有四种凝集素均呈阳性，外根鞘被PNA染色。皮脂腺导管以及皮脂腺导管插入水平的外根鞘也被所有四种凝集素标记。皮脂腺小叶显示成熟皮脂腺细胞的细胞质和膜被PNA和SBA染色。尽管汗腺肿瘤与其相应正常组织相比在凝集素结合方面存在差异，但毛囊皮脂腺肿瘤的凝集素结合模式与毛囊皮脂腺结构相似。
