Schaumburg-Lever G
Department of Dermatology, Eberhard-Karls-Universität, Tübingen, West Germany.
J Invest Dermatol. 1990 Apr;94(4):465-70. doi: 10.1111/1523-1747.ep12874590.
The distribution of carbohydrate residues in keratinocytes of normal epidermis was studied. Normal skin was embedded in Lowicryl. Thin sections were incubated with concanavalin A (Con A), peanut agglutinin (PNA), wheat germ agglutinin (WGA), Ulex europaeus agglutinin I (UEA I), dolichos biflorus agglutinin (DBA), and soybean agglutinin (SBA). A positive reaction in the dermis, in the basal lamina (lamina densa, lamina lucida), intracellularly and within the plasma membrane including the desmosomes was obtained after incubation with Con A and WGA. PNA binding sites were found predominantly in the plasma membrane between the desmosomes. The labeling with Con A, WGA, and PNA was most pronounced in the upper stratum spinosum and granulosum. Incubation with UEA revealed heavy labeling of the keratohyalin granules and the cytoplasm of the corneocytes. Incubation with DBA and SBA revealed weak labeling of the keratinocytes. The study of the distribution of carbohydrate residues in normal epidermis is important, since alterations in this distribution might be linked to autoimmunity or malignant transformation.
研究了正常表皮角质形成细胞中碳水化合物残基的分布。将正常皮肤包埋于低温包埋剂中。薄切片与伴刀豆球蛋白A（Con A）、花生凝集素（PNA）、麦胚凝集素（WGA）、荆豆凝集素I（UEA I）、双花扁豆凝集素（DBA）和大豆凝集素（SBA）一起孵育。用Con A和WGA孵育后，在真皮、基膜（致密层、透明层）、细胞内以及包括桥粒在内的质膜内获得了阳性反应。PNA结合位点主要位于桥粒之间的质膜中。Con A、WGA和PNA的标记在上层棘层和颗粒层最为明显。用UEA孵育显示透明角质颗粒和角质形成细胞的细胞质有大量标记。用DBA和SBA孵育显示角质形成细胞有微弱标记。对正常表皮中碳水化合物残基分布的研究很重要，因为这种分布的改变可能与自身免疫或恶性转化有关。
