Department of Orthopaedic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Spine (Phila Pa 1976). 2011 Jan 15;36(2):103-8. doi: 10.1097/BRS.0b013e3181cb46ba.
an in vitro and in vivo experimental study.
this study was undertaken to evaluate differences in the capability of inducing an implant-centered infection between Staphylococcus aureus and Mycobacterium tuberculosis.
bacterial infection is still one of the most serious and devastating complications after orthopedic implant surgery despite the advent of new antibiotics and treatment methods.
S. aureus and M. tuberculosis were separately cultured with titanium plates. The bacteria colonized on the plates were isolated and cultured on culture medium. They were evaluated and compared by colony-forming units enumeration. Scanning electron microscopy was used to evaluate the difference in the colonization features of the 2 pathogens. In the in vivo experiment, 22 dogs were used to assess the susceptibility to infection after a local bacterial challenge with either S. aureus or M. tuberculosis.
S. aureus showed heavy adhesion and multiplication on the surface of titanium plates in vitro, whereas M. tuberculosis rarely adhered to the surface of the plates. Under scanning electron microscopy, S. aureus colonization was observed: the coccoid was widespread on the surface of the plates but only a few M. tuberculosis cells scattered on the surface of the plates. In in vivo test, the infection rateforthe S. aureus inoculation was higher than that for the M. tuberculosis challenge. The infection rate for the entire test population (n = 44 sites) was 39.58% (19/48). The infection rates were 54.17% (13/24) for the S. aureus challenge and 25% (6/24) for the M. tuberculosis challenge, respectively (P < 0.05).
it is less likely for M. tuberculosis to adhere and form a biofilm on an implant surface than S. aureus. Under otherwise identical conditions, M. tuberculosis contamination following instrumented spine surgery might lead to less occurrence of infection than S. aureus contamination.
一项体外和体内实验研究。
本研究旨在评估金黄色葡萄球菌和结核分枝杆菌诱导种植体中心感染能力的差异。
尽管新抗生素和治疗方法的出现，细菌感染仍然是骨科植入手术后最严重和最具破坏性的并发症之一。
分别用钛板培养金黄色葡萄球菌和结核分枝杆菌。将在平板上定殖的细菌分离并在培养基上培养。通过集落形成单位计数进行评估和比较。扫描电子显微镜用于评估两种病原体定植特征的差异。在体内实验中，22 只狗用于评估局部金黄色葡萄球菌或结核分枝杆菌细菌挑战后感染的易感性。
金黄色葡萄球菌在体外大量粘附和增殖于钛板表面，而结核分枝杆菌很少粘附于平板表面。在扫描电子显微镜下，观察到金黄色葡萄球菌的定植：球菌广泛分布于平板表面，但只有少数结核分枝杆菌细胞散在平板表面。在体内试验中，金黄色葡萄球菌接种的感染率高于结核分枝杆菌挑战的感染率。金黄色葡萄球菌接种的感染率为 39.58%（19/48），整个试验人群（n=44 个部位）的感染率为 54.17%（13/24）。金黄色葡萄球菌和结核分枝杆菌的感染率分别为 25%（6/24）和 54.17%（13/24）（P<0.05）。
结核分枝杆菌在植入物表面粘附和形成生物膜的可能性低于金黄色葡萄球菌。在其他条件相同的情况下，与金黄色葡萄球菌污染相比，脊柱内固定术后结核分枝杆菌污染可能导致感染的发生率更低。
