Department of Physics, University of Gothenburg, Gothenburg, Sweden.
Lab Chip. 2010 Aug 21;10(16):2049-51. doi: 10.1039/c004878j. Epub 2010 Jun 14.
We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.
我们证明了，在 180nm 深的 Si/SiO2 纳米漏斗中限制的 YOYO 标记 DNA 分子的检测发射强度会随着漏斗宽度的变化而显著变化，但不是单调变化。对于定量荧光显微镜和光子预算紧张的实验来说，这种效应可能很重要。
