Department of Neurology, Hannover Medical School, Hanover, Germany.
Neurochem Res. 2010 Sep;35(9):1422-33. doi: 10.1007/s11064-010-0202-7. Epub 2010 Jun 11.
In this study, we investigated the potential of minocycline to influence cuprizone induced demyelination in the grey and white matter. To induce demyelination C57BL/6 mice were fed with cuprizone for up to 6 weeks and were analysed at different timepoints (week 0, 4, 5, 6). Mice treated with minocycline had less demyelination of the cortex and corpus callosum compared with sham treated animals. In the cortex decreased numbers of activated and proliferating microglia were found after 6 weeks of cuprizone feeding, while there were no significant effects for microglial infiltration of the corpus callosum. In addition to the beneficial effects on demyelination, minocycline prevented from motor coordination disturbance as shown in the beam walking test. For astrogliosis and the numbers of OPC and oligodendrocytes no treatment effects were found. In summary, minocycline treatment diminished the course of demyelination in the grey and white matter and prevented disturbances in motor coordination.
在这项研究中，我们研究了米诺环素对铜诱导的灰质和白质脱髓鞘的影响。为了诱导脱髓鞘，C57BL/6 小鼠用铜处理长达 6 周，并在不同时间点（0 周、4 周、5 周、6 周）进行分析。与假处理动物相比，米诺环素治疗的小鼠大脑皮层和胼胝体的脱髓鞘程度较轻。在铜处理 6 周后，发现皮层中活化和增殖的小胶质细胞数量减少，而胼胝体的小胶质细胞浸润没有显著影响。除了对脱髓鞘的有益作用外，米诺环素还防止了运动协调障碍，如在横梁行走测试中所示。对于星形胶质细胞增生和少突胶质细胞前体细胞和少突胶质细胞的数量，没有发现治疗效果。总之，米诺环素治疗减轻了灰质和白质脱髓鞘的过程，并防止了运动协调障碍。
