Department of Ultrasound, Xijing Hospital, Fourth Military Med University, Xi'an, Shann Xi, China.
Mol Imaging Biol. 2011 Apr;13(2):293-302. doi: 10.1007/s11307-010-0350-9.
We hypothesized that post-myocardial ischemia-reperfusion (I/R) remodeling associated matrix metalloproteinase-2 (MMP(2)) activation could be detected by using novel MMP(2) targeted ultrasound imaging.
We study the combination of MMP(2)-targeted microbubbles (TMB(2)) and control microbubbles with myocardium in 1 week post-I/R rats.
In in vitro studies, TMB(2) significantly bound within the risk area (RA) of 1-week post-I/R myocardial sections while rare binding was observed in the control area (CA). In in vivo studies, increased focal retention of TMB(2) was observed within the RA, with the higher myocardial video intensity (RA 42.85 ± 20.12 dB versus CA 25.85 ± 13.40 dB, p < 0.01). However, there was no difference of control microbubble retention in both CA and RA.
A targeted ultrasound contrast imaging approach that employs novel TMB(2) has the potential to provide a less-invasive, higher-resolution technique for in vivo localization of MMP(2) activation and tracking of MMP-mediated post-I/R remodeling.
我们假设通过使用新型 MMP（2）靶向超声成像，可以检测到心肌缺血再灌注（I/R）后重塑相关的基质金属蛋白酶-2（MMP（2））激活。
我们研究了 MMP（2）靶向微泡（TMB（2））与心肌在 I/R 后 1 周的对照微泡的组合。
在体外研究中，TMB（2）在 1 周 post-I/R 心肌切片的风险区（RA）内有明显结合，而在对照区（CA）内则很少有结合。在体内研究中，TMB（2）在 RA 内的局灶性保留增加，心肌视频强度更高（RA 42.85±20.12dB 与 CA 25.85±13.40dB，p<0.01）。然而，在 CA 和 RA 中，对照微泡的保留没有差异。
一种采用新型 TMB（2）的靶向超声对比成像方法具有提供一种微创、高分辨率的 MMP（2）激活定位和 MMP 介导的 post-I/R 重塑跟踪的技术潜力。
