Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, UFSC, Florianópolis, Santa Catarina, Brazil.
Appl Biochem Biotechnol. 2010 Nov;162(7):1881-8. doi: 10.1007/s12010-010-8966-4. Epub 2010 Jun 11.
The main objective of this work was the isolation and screening of microorganisms with potential for producing lipases for the synthesis of fatty esters as well as evaluating the specificity of the enzymes produced, using different alcohols (methanol, ethanol, n-propanol, and butanol) and fatty acids (oleic and lauric acids) as substrates. Promising biocatalysts for organic synthesis were obtained in this work. The isolated strains 69F and 161Y showed ability to efficiently catalyze the reaction for production of n-propyl oleate. Other strains can also be considered of potential interest, as 74F, 111Y, and 186Y. The future development of production using different substrates could result in cheap crude lipase of high importance to industrial applicability.
本工作的主要目的是分离和筛选具有产生脂肪酶潜力的微生物,用于合成脂肪酸酯,同时评估不同醇(甲醇、乙醇、正丙醇和正丁醇)和脂肪酸(油酸和月桂酸)作为底物时所产生的酶的特异性。在这项工作中获得了有前途的有机合成用生物催化剂。分离得到的菌株 69F 和 161Y 显示出有效催化生产正丙基油酸酯反应的能力。其他菌株也可能具有潜在的应用价值,如 74F、111Y 和 186Y。未来使用不同底物进行生产的开发可能会产生廉价的粗脂肪酶,这对工业应用具有重要意义。