离心收缩会导致缺乏 dystrophin 的狗出现快速等长扭矩下降。
Eccentric contractions induce rapid isometric torque drop in dystrophin-deficient dogs.
机构信息
Wake Forest University Health Sciences, Institute for Regenerative Medicine, 391 Technology Way, Winston-Salem, North Carolina 27101, USA.
出版信息
Muscle Nerve. 2010 Jul;42(1):130-2. doi: 10.1002/mus.21699.
Abstract
We tested the hypothesis that eccentric contractions (ECCs) rapidly induce greater-than-normal isometric torque drop in dystrophin-deficient golden retriever muscular dystrophy (GRMD) muscles. ECCs were imposed by forcibly stretching activated muscles. The results indicate that isometric torque drop was greater in GRMD versus controls (P < 0.0001). Our findings support the hypothesis that ECCs induce greater-than-normal isometric torque drop in GRMD muscles. The magnitude of ECC-induced isometric torque loss may be an ideal clinical endpoint in the GRMD model.
摘要
我们验证了这样一个假设，即偏心收缩（ECC）会迅速导致缺乏肌营养不良蛋白的金猎犬肌营养不良症（GRMD）肌肉的等长扭矩下降超过正常水平。ECC 是通过强制拉伸激活的肌肉来实现的。结果表明，GRMD 组的等长扭矩下降大于对照组（P < 0.0001）。我们的发现支持这样一个假设，即 ECC 会导致 GRMD 肌肉的等长扭矩下降超过正常水平。ECC 引起的等长扭矩损失的幅度可能是 GRMD 模型中的一个理想的临床终点。
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2
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