Vlasova A L, Preobrazhenskaia M E, Khoroshko N D
Biull Eksp Biol Med. 1991 Mar;111(3):251-4.
The presence of neutral soluble alpha-D-mannosidase activity was shown in human granulocytes. For detection of the enzyme different methods were used: addition of stabilizing agents; sorption of acid alpha-D-mannosidase on concanavalin A-sepharose; inhibition of acid alpha-D-mannosidase; determination of neutral alpha-D-mannosidase in granulocytes of patients with inherited defect of acid alpha-D-mannosidase (mannosidosis). The specific activity of neutral alpha-D-mannosidase in granulocytes of donors calculated in nmol/min/mg of protein was near to the activity in lymphocytes. However the activity in granulocytes calculated in nmol/min/10(8) of cells was approximately 3 times lower than that in lymphocytes. The activity of neutral alpha-D-mannosidase in immature myeloid cells of a patient with chronic myeloid leukaemia was 10 times higher than in natural granulocytes of the same patient. This high activity may be in connection with the process of cell differentiation or the result of malignant transformation.
在人类粒细胞中发现了中性可溶性α-D-甘露糖苷酶活性。为检测该酶，使用了不同方法：添加稳定剂；将酸性α-D-甘露糖苷酶吸附于伴刀豆球蛋白A-琼脂糖；抑制酸性α-D-甘露糖苷酶；测定酸性α-D-甘露糖苷酶遗传性缺陷（甘露糖苷贮积症）患者粒细胞中的中性α-D-甘露糖苷酶。以nmol/分钟/毫克蛋白质计算，供体粒细胞中中性α-D-甘露糖苷酶的比活性接近淋巴细胞中的活性。然而，以nmol/分钟/10⁸个细胞计算，粒细胞中的活性比淋巴细胞中的活性低约3倍。一名慢性髓性白血病患者未成熟髓细胞中中性α-D-甘露糖苷酶的活性比该患者天然粒细胞中的活性高10倍。这种高活性可能与细胞分化过程有关，或是恶性转化的结果。
