Vlasova A L, Ushakova N A, Preobrazhenskaia M E
Biokhimiia. 1991 Aug;56(8):1479-87.
The presence of neutral alpha-mannosidase activity in normal and pathological lymphoid cells has been demonstrated. The specific activities of the enzyme in different cell types were similar with the exception of B-cells from B-CLL patients when it was a little higher. The activity of acid alpha-mannosidase was also determined in these lymphoid cells. The neutral to acid alpha-mannosidase activity ratio was different in B- and T-cells: in the former neutral alpha-mannosidase activity prevailed, whereas in the latter the predominance of acid alpha-mannosidase activity was apparent. Neutral alpha-mannosidases from pathological B- and T-cells were partially purified and their properties were investigated. In both cell types the enzyme was localized in the cytosol, was very labile and could be stabilized with Mn2+ and dithiothreitol. The enzyme was activated by Co2+ and inhibited by Zn2+ and EDTA. Swainsonine inhibited the B-cell neutral alpha-mannosidase somewhat more strongly in comparison with the T-cell enzyme.
已证实在正常和病理淋巴样细胞中存在中性α-甘露糖苷酶活性。除了B - CLL患者的B细胞中该酶的比活性略高外，不同细胞类型中该酶的比活性相似。还测定了这些淋巴样细胞中酸性α-甘露糖苷酶的活性。B细胞和T细胞中中性与酸性α-甘露糖苷酶活性之比不同：在前者中中性α-甘露糖苷酶活性占优势，而在后者中酸性α-甘露糖苷酶活性占优势明显。对来自病理B细胞和T细胞的中性α-甘露糖苷酶进行了部分纯化并研究了其性质。在两种细胞类型中，该酶都定位于胞质溶胶中，非常不稳定，并且可以用Mn2 +和二硫苏糖醇稳定。该酶被Co2 +激活，被Zn2 +和EDTA抑制。与T细胞酶相比，苦马豆素对B细胞中性α-甘露糖苷酶的抑制作用略强。
