Kobe Advanced ICT Research Center, National Institute of Information and Communications Technology, Japan.
Genes Cells. 2010 Jun;15(7):661-9. doi: 10.1111/j.1365-2443.2010.01415.x. Epub 2010 Jun 7.
The nucleoporin Nup98 is an essential component of the nuclear pore complex. This peripheral nucleoporin with its Gly-Leu-Phe-Gly (GLFG) repeat domain contributes to nuclear-cytoplasmic trafficking, including mRNA export. In addition, accumulating studies indicate that Nup98 plays roles in several important biological events such as gene expression, mitotic checkpoint, and pathogenesis. Nup98 is well conserved among organisms belonging to the fungi and animal kingdoms. These kingdoms belong to the eukaryotic supergroup Opisthokonta. However, there is considerable diversity in the Nup98 orthologs expressed in organisms belonging to other eukaryotic supergroups. Intriguingly, in ciliates, a unicellular organism having two functionally distinct nuclei, GLFG-Nup98 is present in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus, and these different Nup98s participate in a nucleus-selective transport mechanism. In this review, we focus on Nup98 function and discuss how this nucleoporin has evolved in eukaryotic kingdoms.
核孔蛋白 Nup98 是核孔复合物的必需组成部分。这种具有 Gly-Leu-Phe-Gly (GLFG) 重复结构域的外周核孔蛋白有助于核质运输，包括 mRNA 输出。此外，越来越多的研究表明，Nup98 在几个重要的生物学事件中发挥作用，如基因表达、有丝分裂检查点和发病机制。Nup98 在真菌和动物王国的生物体中高度保守。这些王国属于真核超组后生动物。然而，在属于其他真核超组的生物体中表达的 Nup98 同源物存在相当大的多样性。有趣的是，在纤毛类生物中，一种具有两个功能不同核的单细胞生物，GLFG-Nup98 存在于一个核中，另一个核中存在一个不同的 Nup98 同源物，并且这些不同的 Nup98 参与核选择性运输机制。在这篇综述中，我们重点介绍了 Nup98 的功能，并讨论了这种核孔蛋白在真核生物王国中的进化方式。
