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甘油-3-磷酸O-酰基转移酶是家蚕中PBAN诱导的性信息素生物合成所必需的。

Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori.

作者信息

Du Mengfang, Liu Xiaoguang, Liu Xiaoming, Yin Xinming, Han Shuangyin, Song Qisheng, An Shiheng

机构信息

State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002 P.R. China.

Translational Research Center, Zhengzhou University People's Hospital, Zhengzhou 450003 P.R. China.

出版信息

Sci Rep. 2015 Jan 29;5:8110. doi: 10.1038/srep08110.

DOI:10.1038/srep08110
PMID:25630665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5389035/
Abstract

Female moths employ their own pheromone blends as a communicational medium in mating behavior. The biosynthesis and release of sex pheromone in female moths are regulated by pheromone biosynthesis activating neuropeptide (PBAN) and the corresponding action of PBAN has been well elucidated in Bombyx mori. However, very little is known about the molecular mechanism regarding the biosynthesis of sex pheromone precursor. In this study, quantitative proteomics was utilized to comprehensively elucidate the expression dynamics of pheromone glands (PGs) during development. Proteomic analysis revealed a serial of differentially expressed sex pheromone biosynthesis-associated proteins at the different time points of B. mori development. Most interestingly B. mori glycerol-3-phosphate O-acyltransferase (BmGPAT) was found to be expressed during the key periods of sex pheromone biosynthesis. RNAi knockdown of BmGPAT confirmed the important function of this protein in the biosynthesis of sex pheromone precursor, triacylglcerol (TAG), and subsequently PBAN-induced production of sex pheromone, bombykol. Behavioral analysis showed that RNAi knockdown of GPAT significantly impaired the ability of females to attract males. Our findings indicate that GPAT acts to regulate the biosynthesis of sex pheromone precursor, TAG, thus influencing PBAN-induced sex pheromone production and subsequent mating behavior.

摘要

雌性蛾类利用自身的性信息素混合物作为交配行为中的通讯媒介。雌性蛾类中性信息素的生物合成和释放受性信息素生物合成激活神经肽（PBAN）调控，且PBAN在家蚕中的相应作用已得到充分阐明。然而，关于性信息素前体生物合成的分子机制却知之甚少。在本研究中，利用定量蛋白质组学全面阐明了发育过程中信息素腺体（PGs）的表达动态。蛋白质组学分析揭示了家蚕发育不同时间点一系列差异表达的与性信息素生物合成相关的蛋白质。最有趣的是，发现家蚕甘油-3-磷酸O-酰基转移酶（BmGPAT）在性信息素生物合成的关键时期表达。对BmGPAT进行RNA干扰敲低证实了该蛋白在性信息素前体三酰甘油（TAG）生物合成以及随后PBAN诱导的性信息素蚕蛾醇产生中的重要作用。行为分析表明，对GPAT进行RNA干扰敲低显著损害了雌性吸引雄性的能力。我们的研究结果表明，GPAT在调节性信息素前体TAG的生物合成中发挥作用，从而影响PBAN诱导的性信息素产生及随后的交配行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/ae920d6d0277/srep08110-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/385c35af9d30/srep08110-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/04c85f234f7e/srep08110-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/17d580083d4b/srep08110-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/28c9594850f6/srep08110-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/adc7d2f5593f/srep08110-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/8168a9bc4347/srep08110-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/ae920d6d0277/srep08110-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/385c35af9d30/srep08110-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/04c85f234f7e/srep08110-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/17d580083d4b/srep08110-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/28c9594850f6/srep08110-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/adc7d2f5593f/srep08110-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/8168a9bc4347/srep08110-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20e8/5389035/ae920d6d0277/srep08110-f7.jpg

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