Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
J Ethnopharmacol. 2010 Jul 20;130(2):363-8. doi: 10.1016/j.jep.2010.05.019. Epub 2010 Jun 1.
Radix Bupleuri, one of the most frequently prescribed crude herbs in traditional Chinese medicine, has been used for centuries to treat inflammatory diseases. However, little is known about the therapeutic mechanisms of crude polysaccharides (BPs) isolated from the roots of Bupleurum smithii var. parvifolium. Macrophages play important roles in inflammatory diseases such as systemic lupus erythematosus (SLE). The purpose of the present work was to investigate immunomodulative effects of Bupleurum polysaccharides on murine peritoneal macrophages.
BALB/c mice were administered intragastrically with Bupleurum polysaccharides 20, 40, and 80 mg kg(-1) day(-1), or prednisone 3 mg kg(-1) day(-1) or levamisole 25 mgk g(-1) day(-1) from day 0 to day 6. Peritoneal macrophages were isolated 5 days after intraperitoneal injection of 1 mL 5% sodium thioglycollate. Phagocytic functions of macrophages were studied; cytokines concentrations in the culture supernatants were determined by enzyme-linked immunosorbent assay and the secretion of nitric oxide (NO) was quantified by Griess reaction.
Treatment with BPs enhanced phagocytic functions of macrophages (phagocytosis of apoptotic thymocytes, IgG-opsonized sheep red blood cells and chicken red blood cells) and inhibited LPS-induced productions of NO and proinflammatory cytokines (interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha).
Bupleurum polysaccharides up-regulated phagocytic activities but inhibited LPS-induced productions of proinflammatory mediators. These data suggested that at least part of the traditional beneficial effects of Bupleurum on inflammatory diseases could be ascribed to the immunomodulatory effects of Bupleurum polysaccharides on macrophages.
柴胡是中药中最常用的草药之一，几个世纪以来一直用于治疗炎症性疾病。然而，对于从小叶黑柴胡根中分离得到的粗多糖（BPs）的治疗机制知之甚少。巨噬细胞在系统性红斑狼疮（SLE）等炎症性疾病中发挥重要作用。本研究旨在探讨柴胡多糖对小鼠腹腔巨噬细胞的免疫调节作用。
BALB/c 小鼠从第 0 天到第 6 天每天灌胃给予 20、40 和 80mg/kg 柴胡多糖，或给予 3mg/kg 泼尼松或 25mg/kg 左旋咪唑。腹腔内注射 1ml5%巯基乙氨酸钠 5 天后分离腹腔巨噬细胞。研究巨噬细胞的吞噬功能；通过酶联免疫吸附试验测定培养上清液中细胞因子的浓度，通过 Griess 反应定量测定一氧化氮（NO）的分泌。
BP 治疗增强了巨噬细胞的吞噬功能（对凋亡胸腺细胞、IgG 调理的绵羊红细胞和鸡红细胞的吞噬作用），并抑制了 LPS 诱导的 NO 和促炎细胞因子（白细胞介素-1β、白细胞介素-6 和肿瘤坏死因子-α）的产生。
柴胡多糖上调了吞噬活性，但抑制了 LPS 诱导的促炎介质的产生。这些数据表明，柴胡对炎症性疾病的部分传统有益作用至少可以归因于柴胡多糖对巨噬细胞的免疫调节作用。
Zotero 插件