Department of Pharmacy, Henan University of Chinese Medicine.
The Engineering and Technology Center for Chinese Medicine Development of Henan Province.
J Tradit Chin Med. 2022 Aug;42(4):546-555. doi: 10.19852/j.cnki.jtcm.20220516.003.
to explore the nature of ascending-descending-floating-sinking of Traditional Chinese Medicine on normal rats using gonadotropin-releasing hormone (GnRh).
Normal male Sprague-Dawley rats were orally administered six floating Chinese medicines and seven sinking Chinese medicines for 14 d. then, GnRh and relevant indicators were detected. Initially, the different effects of floating and sinking drugs on the body were explored. To verify the effects of floating and sinking drugs on the body, normal rats were orally administered Mahuang (Herba Ephedra Sinica) and Tinglizi (Semen Lepidii Apetali) for 14 d. Then, GnRh antagonists were administered. We observed the changes of relevant indicators and clarified the correlation between GnRh and the laws of floating and sinking.
Floating Chinese medicines significantly increased the testicular coefficients; GnRh level in the serum; the protein level of GnRh receptor (GnRhR) in the testis; and the mRNA levels of androgen receptor (AR), small C-terminal domain phosphatase (SCP) 3, SCP2, SYCE1, SMC1B, SMC3, and Rec8 (P < 0.01 or < 0.05). Sinking Chinese medicines did not react similarly, while GnRh antagonists blocked the regulatory effect of Mahuang (Herba Ephedra Sinica) but did not affect Tinglizi (Semen Lepidii Apetali).
GnRh may be closely related to the nature of ascending-descending-floating-sinking of Chinese medicine. Floating Chinese medicines may promote their medicinal properties by regulating GnRH level; however, sinking Chinese medicines did not affect GnRh level.
利用促性腺激素释放激素（GnRH）探讨中药升降浮沉对正常大鼠的作用机制。
将正常雄性 Sprague-Dawley 大鼠分别灌胃 6 种升浮中药和 7 种沉降中药 14 d，检测 GnRh 及相关指标，初步探讨升浮、沉降药对机体的不同影响；为验证升浮沉降药对机体的影响，分别灌胃麻黄（Herba Ephedra Sinica）和萹蓄（Semen Lepidii Apetali）14 d，再给予 GnRH 拮抗剂，观察相关指标的变化，阐明 GnRh 与升降浮沉规律的相关性。
升浮中药可显著增加睾丸系数；血清 GnRh 水平；睾丸 GnRh 受体（GnRhR）蛋白水平；雄激素受体（AR）、小 C 端磷酸酶 3（SCP）3、SCP2、SYCE1、SMC1B、SMC3、Rec8mRNA 水平（P<0.01 或 P<0.05）；沉降中药则无类似反应，GnRh 拮抗剂可阻断麻黄（Herba Ephedra Sinica）的调节作用，但对萹蓄（Semen Lepidii Apetali）无影响。
GnRh 可能与中药升降浮沉的性质密切相关，升浮中药可能通过调节 GnRH 水平来促进其药效，而沉降中药不影响 GnRh 水平。
